Universität zu Köln

Wu, Zhiqun, Nakamura, Makoto, Krauss, Joachim K., Schwabe, Kerstin and Joh, Nadine (2018). Intracranial rat glioma model for tumor resection and local treatment. J. Neurosci. Methods, 299. S. 1 - 8. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 1872-678X

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Abstract

Background: Although tumor resection is among the most important prognostic factors, high grade gliomas regrow in most cases. Also, resection of glial tumors in eloquent brain regions is not or only partially possible. Despite these severe restraints, however, only a few in-vivo models have been established to investigate tumor recurrence and local treatment. Here we characterize the intracranial BT4Ca rat glioma as a model for these aspects. New method: BT4Ca cells were stereotaxically implanted into the frontal cortex of BDIX rats. Rats were than allocated to (1) a control group, which received no further treatment; (2) a catheter group, where a catheter was implanted for repeated microinjection of vehicle every 3rd day as catheter-control; (3) a resection group, where the tumor was microsurgically removed eight days after cell injection. Postoperatively, survival time, weight and general health condition were scored and the tumor size was histologically assessed. Results: Injection of BT4Ca cells induced fast-growing tumors with a mean survival time of 16 days in the control and catheter groups. Resection significantly prolonged survival time whereby the tumor regrew in all rats. Tumor size was similar between all groups. Comparison with existing method(s): We here present a robust and reliable intracranial rat glioma model, which is suitable to simulate tumor recurrence after surgical resection and local treatment. Importantly, this model does not require advanced imaging or elaborate surgical techniques. Conclusions: The intracranial BT4Ca glioma model appears to be a feasible tool to investigate tumor recurrence after resection and to test local treatment. (C) 2018 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wu, Zhiqun UNSPECIFIED UNSPECIFIED UNSPECIFIED Nakamura, Makoto UNSPECIFIED UNSPECIFIED UNSPECIFIED Krauss, Joachim K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwabe, Kerstin UNSPECIFIED UNSPECIFIED UNSPECIFIED Joh, Nadine UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-190533
DOI:	10.1016/j.jneumeth.2018.02.002
Journal or Publication Title:	J. Neurosci. Methods
Volume:	299
Page Range:	S. 1 - 8
Date:	2018
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	1872-678X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CONVECTION-ENHANCED DELIVERY; MEDIAL PREFRONTAL CORTEX; GROSS TOTAL RESECTION; GLIOBLASTOMA-MULTIFORME; ADJUVANT TEMOZOLOMIDE; BRAIN-TUMORS; SURVIVAL; RADIOTHERAPY; CONCOMITANT; STANDARDS Multiple languages Biochemical Research Methods; Neurosciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19053