Universität zu Köln

Pahlavan, Sara ORCID: 0000-0002-8854-2626, Tousi, Marziyeh Shalchi, Ayyari, Mahdi, Alirezalu, Abolfazl ORCID: 0000-0002-5882-8981, Ansari, Hassan, Saric, Tomo and Baharvand, Hossein (2018). Effects of hawthorn (Crataegus pentagyna) leaf extract on electrophysiologic properties of cardiomyocytes derived from human cardiac arrhythmia-specific induced pluripotent stem cells. Faseb J., 32 (3). S. 1440 - 1452. BETHESDA: FEDERATION AMER SOC EXP BIOL. ISSN 1530-6860

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Abstract

Cardiac arrhythmias are major life-threatening conditions. The landmark discovery of induced pluripotent stem cells has provided a promising in vitro system for modeling hereditary cardiac arrhythmias as well as drug development and toxicity testing. Nowadays, nutraceuticals are frequently used as supplements for cardiovascular therapy. Here we studied the cardiac effects of hawthorn (Crataegus pentagyna) leaf extract using cardiomyocytes (CMs) differentiated from healthy human embryonic stem cells, long QT syndrome type 2 (LQTS2), and catecholaminergic polymorphic ventricular tachycardia type 1 (CPVT1) patient-specific induced pluripotent stem cells. The hydroalcoholic extract resulted in a dose-dependent negative chronotropic effect in all CM preparations leading to a significant reduction at 1000 mu g/ml. This was accompanied by prolongation of field potential durations, although with different magnitudes in CMs from different human embryonic stem cell and iPSC lines. Hawthorn further prolonged field potential durations in LQTS2 CMs but reduced the beating frequencies and occurrence of immature field potentials triggered by beta(1)-adrenergic stimulation in CPVT1 CMs at 300 and 1000 mu g/ml. Furthermore, isoquercetin and vitexin flavonoids significantly slowed down isoproterenol (5 mu M)-induced beating frequencies at 3 and 10 mu g/ml. Therefore, C. pentagyna leaf extract and its isoquercetin and vitexin flavonoids may be introduced as a novel nutraceutical with antiarrhythmic potential for CPVT1 patients.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Pahlavan, Sara UNSPECIFIED orcid.org/0000-0002-8854-2626 UNSPECIFIED Tousi, Marziyeh Shalchi UNSPECIFIED UNSPECIFIED UNSPECIFIED Ayyari, Mahdi UNSPECIFIED UNSPECIFIED UNSPECIFIED Alirezalu, Abolfazl UNSPECIFIED orcid.org/0000-0002-5882-8981 UNSPECIFIED Ansari, Hassan UNSPECIFIED UNSPECIFIED UNSPECIFIED Saric, Tomo UNSPECIFIED UNSPECIFIED UNSPECIFIED Baharvand, Hossein UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-194375
DOI:	10.1096/fj.201700494RR
Journal or Publication Title:	Faseb J.
Volume:	32
Number:	3
Page Range:	S. 1440 - 1452
Date:	2018
Publisher:	FEDERATION AMER SOC EXP BIOL
Place of Publication:	BETHESDA
ISSN:	1530-6860
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POLYMORPHIC VENTRICULAR-TACHYCARDIA; HEART-FAILURE; RAT MODEL; OXYACANTHA; PATIENT; DIFFERENTIATION; COMPLEMENTARY; PHARMACOLOGY; PROGRESSION; GENERATION Multiple languages Biochemistry & Molecular Biology; Biology; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19437