Universität zu Köln

Lockau, Hannah, Neuschmelting, Volker, Ogirala, Anuja, Vilaseca, Antoni ORCID: 0000-0003-0160-620X and Grimm, Jan (2018). Dynamic F-18-FDG PET Lymphography for In Vivo Identification of Lymph Node Metastases in Murine Melanoma. J. Nucl. Med., 59 (2). S. 210 - 216. RESTON: SOC NUCLEAR MEDICINE INC. ISSN 1535-5667

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Abstract

Positron lymphography using F-18-FDG followed by Cerenkov-guided resection of lymph nodes in healthy mice has previously been introduced by our group. Our aim in this study was to further assess the technique's potential beyond merely localizing sentinel lymph nodes. We now aimed to evaluate the potential of positron lymphography to characterize the nodes with respect to their tumor status in order to identify metastatic lymph nodes. We explored whether metastatic nodes could be distinguished from normal nodes via dynamic F-18-FDG lymphography, to then be resected under Cerenkov imaging guidance. Methods: A murine melanoma cell line highly metastatic to lymph nodes (B16F10) was implanted subcutaneously on the dorsal hind paw of C57 mice while the tumor-free contralateral leg served as an intraindividual control. A model of reactive lymph nodes after concanavalin A challenge served as an additional control to provide nonmalignant inflammatory lymphadenopathy. Dynamic PET/CT imaging was performed immediately after injection of F-18-FDG around the tumor or intracutaneously in the contralateral footpad. Furthermore, PET/CT and Cerenkov studies were performed repeatedly over time to follow the course of metastatic spread. In selected mice, popliteal lymph nodes underwent Cerenkov luminescence imaging. Hematoxylin and eosin staining was done to verify the presence of lymphatic melanoma infiltration. Results: Positron lymphography using F-18-FDG was successfully performed on tumor-bearing and non-tumor-bearing mice, as well as on controls bearing sites of inflammation; the results clearly identified the sentinel lymph node basin and delineated the lymphatic drainage. Significantly prolonged retention of activity was evident in metastatic nodes as compared with controls without tumor. On the basis of these results, the contrast in detection and identification of metastatic lymph nodes was distinct and could be used for guided lymph node resection, such as by using Cerenkov luminescence imaging. However, retention after F-18-FDG lymphography was also seen in acute inflammatory lymphadenopathy. Conclusion: In a tumor model, significantly longer retention of the radiotracer during F-18-FDG lymphography was seen in metastatic than nonmetastatic lymph nodes, allowing for differentiation between the two and for selective resection of tumor-bearing nodes using Cerenkov imaging. Inflammation can be better differentiated in a subacute state.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lockau, Hannah UNSPECIFIED UNSPECIFIED UNSPECIFIED Neuschmelting, Volker UNSPECIFIED UNSPECIFIED UNSPECIFIED Ogirala, Anuja UNSPECIFIED UNSPECIFIED UNSPECIFIED Vilaseca, Antoni UNSPECIFIED orcid.org/0000-0003-0160-620X UNSPECIFIED Grimm, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-197996
DOI:	10.2967/jnumed.117.196303
Journal or Publication Title:	J. Nucl. Med.
Volume:	59
Number:	2
Page Range:	S. 210 - 216
Date:	2018
Publisher:	SOC NUCLEAR MEDICINE INC
Place of Publication:	RESTON
ISSN:	1535-5667
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CUTANEOUS MELANOMA; TUMOR; BIOPSY; LYMPHANGIOGENESIS; INFLAMMATION; TRACER; EXPERIENCE; CANCER Multiple languages Radiology, Nuclear Medicine & Medical Imaging Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19799