Universität zu Köln

Roma, Leticia Prates, Deponte, Marcel ORCID: 0000-0003-2141-917X, Riemer, Jan and Morgan, Bruce (2018). Mechanisms and Applications of Redox-Sensitive Green Fluorescent Protein-Based Hydrogen Peroxide Probes. Antioxid. Redox Signal., 29 (6). S. 552 - 569. NEW ROCHELLE: MARY ANN LIEBERT, INC. ISSN 1557-7716

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Abstract

Significance: Genetically encoded hydrogen peroxide (H2O2) sensors, based on fusions between thiol peroxidases and redox-sensitive green fluorescent protein 2 (roGFP2), have dramatically broadened the available toolbox for monitoring cellular H2O2 changes. Recent Advances: Recently developed peroxiredoxin-based probes such as roGFP2-Tsa2 Delta C-R offer considerably improved H2O2 sensitivity compared with previously available genetically encoded sensors and now permit dynamic, real-time, monitoring of changes in endogenous H2O2 levels. Critical Issues: The correct understanding and interpretation of probe read-outs is crucial for their meaningful use. We discuss probe mechanisms, potential pitfalls, and best practices for application and interpretation of probe responses and highlight where gaps in our knowledge remain. Future Directions: The full potential of the newly available sensors remains far from being fully realized and exploited. We discuss how the ability to monitor basal H2O2 levels in real time now allows us to re-visit long-held ideas in redox biology such as the response to ischemia-reperfusion and hypoxia-induced reactive oxygen species production. Further, recently proposed circadian cycles of peroxiredoxin hyperoxidation might now be rigorously tested. Beyond their application as H2O2 probes, roGFP2-based H2O2 sensors hold exciting potential for studying thiol peroxidase mechanisms, inactivation properties, and the impact of post-translational modifications, in vivo. Antioxid. Redox Signal. 00, 000-000.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Roma, Leticia Prates UNSPECIFIED UNSPECIFIED UNSPECIFIED Deponte, Marcel UNSPECIFIED orcid.org/0000-0003-2141-917X UNSPECIFIED Riemer, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Morgan, Bruce UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-199002
DOI:	10.1089/ars.2017.7449
Journal or Publication Title:	Antioxid. Redox Signal.
Volume:	29
Number:	6
Page Range:	S. 552 - 569
Date:	2018
Publisher:	MARY ANN LIEBERT, INC
Place of Publication:	NEW ROCHELLE
ISSN:	1557-7716
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language OXIDATIVE STRESS; LIFE-SPAN; TRANSCRIPTION FACTOR; 2-CYS PEROXIREDOXIN; THIOREDOXIN PEROXIDASE; GENE-EXPRESSION; THIOL SWITCHES; NUCLEAR EXPORT; LIVING CELLS; H2O2 Multiple languages Biochemistry & Molecular Biology; Endocrinology & Metabolism Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19900