Baumgart, Meike ORCID: 0000-0002-9874-1151, Unthan, Simon, Kloss, Ramona, Radek, Andreas, Polen, Tino ORCID: 0000-0002-0065-3007, Tenhaef, Niklas ORCID: 0000-0002-9375-4156, Mueller, Moritz Fabian, Kueberl, Andreas, Siebert, Daniel, Bruehl, Natalie, Marin, Kay, Hans, Stephan, Kraemer, Reinhard, Bott, Michael ORCID: 0000-0002-4701-8254, Kalinowski, Joern, Wiechert, Wolfgang ORCID: 0000-0001-8501-0694, Seibold, Gerd ORCID: 0000-0002-9823-8134, Frunzke, Julia ORCID: 0000-0001-6209-7950, Rueckert, Christian, Wendisch, Volker F. ORCID: 0000-0003-3473-0012 and Noack, Stephan ORCID: 0000-0001-9784-3626 (2018). Corynebacterium glutamicum Chassis C1*: Building and Testing a Novel Platform Host for Synthetic Biology and Industrial Biotechnology. ACS Synth. Biol., 7 (1). S. 132 - 145. WASHINGTON: AMER CHEMICAL SOC. ISSN 2161-5063

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Abstract

Targeted top-down strategies for genome reduction are considered to have a high potential for providing robust basic strains for synthetic biology and industrial biotechnology. Recently, we created a library of 26 genome-reduced strains of Corynebacterium glutamicum carrying broad deletions in single gene clusters and showing wild-type-like biological fitness. Here, we proceeded with combinatorial deletions of these irrelevant gene clusters in two parallel orders, and the resulting library of 28 strains was characterized under various environmental conditions. The final chassis strain C1* carries a genome reduction of 13.4% (412 deleted genes) and shows wild-type-like growth behavior in defined medium with D-glucose as carbon and energy source. Moreover, C1* proves to be robust against several stresses (including oxygen limitation) and shows long-term growth stability under defined and complex medium conditions. In addition to providing a novel prokaryotic chassis strain, our results comprise a large strain library and a revised genome annotation list, which will be valuable sources for future systemic studies of C. glutamicum.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Baumgart, MeikeUNSPECIFIEDorcid.org/0000-0002-9874-1151UNSPECIFIED
Unthan, SimonUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kloss, RamonaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Radek, AndreasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Polen, TinoUNSPECIFIEDorcid.org/0000-0002-0065-3007UNSPECIFIED
Tenhaef, NiklasUNSPECIFIEDorcid.org/0000-0002-9375-4156UNSPECIFIED
Mueller, Moritz FabianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kueberl, AndreasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Siebert, DanielUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bruehl, NatalieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Marin, KayUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hans, StephanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kraemer, ReinhardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bott, MichaelUNSPECIFIEDorcid.org/0000-0002-4701-8254UNSPECIFIED
Kalinowski, JoernUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wiechert, WolfgangUNSPECIFIEDorcid.org/0000-0001-8501-0694UNSPECIFIED
Seibold, GerdUNSPECIFIEDorcid.org/0000-0002-9823-8134UNSPECIFIED
Frunzke, JuliaUNSPECIFIEDorcid.org/0000-0001-6209-7950UNSPECIFIED
Rueckert, ChristianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wendisch, Volker F.UNSPECIFIEDorcid.org/0000-0003-3473-0012UNSPECIFIED
Noack, StephanUNSPECIFIEDorcid.org/0000-0001-9784-3626UNSPECIFIED
URN: urn:nbn:de:hbz:38-199935
DOI: 10.1021/acssynbio.7b00261
Journal or Publication Title: ACS Synth. Biol.
Volume: 7
Number: 1
Page Range: S. 132 - 145
Date: 2018
Publisher: AMER CHEMICAL SOC
Place of Publication: WASHINGTON
ISSN: 2161-5063
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ESCHERICHIA-COLI; TRANSCRIPTIONAL REGULATOR; ACETATE METABOLISM; MOLECULAR ANALYSIS; GENOME SEQUENCE; GENE-EXPRESSION; GROWTH; RAMA; STRAIN; QUANTIFICATIONMultiple languages
Biochemical Research MethodsMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/19993

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