Dijkhuizen, Laura W., Brouwer, Paul, Bolhuis, Henk ORCID: 0000-0002-4772-1898, Reichart, Gert-Jan ORCID: 0000-0002-7256-2243, Koppers, Nils, Huettel, Bruno, Bolger, Anthony M., Li, Fay-Wei ORCID: 0000-0002-0076-0152, Cheng, Shifeng, Liu, Xin ORCID: 0000-0003-3256-2940, Wong, Gane Ka-Shu, Pryer, Kathleen, Weber, Andreas, Braeutigam, Andrea and Schluepmann, Henriette ORCID: 0000-0001-6171-3029 (2018). Is there foul play in the leaf pocket? The metagenome of floating fern Azolla reveals endophytes that do not fix N-2 but may denitrify. New Phytol., 217 (1). S. 453 - 467. HOBOKEN: WILEY. ISSN 1469-8137

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Abstract

Dinitrogen fixation by Nostoc azollae residing in specialized leaf pockets supports prolific growth of the floating fern Azolla filiculoides. To evaluate contributions by further microorganisms, the A. filiculoides microbiome and nitrogen metabolism in bacteria persistently associated with Azolla ferns were characterized. A metagenomic approach was taken complemented by detection of N2O released and nitrogen isotope determinations of fern biomass. Ribosomal RNA genes in sequenced DNA of natural ferns, their enriched leaf pockets and water filtrate from the surrounding ditch established that bacteria of A. filiculoides differed entirely from surrounding water and revealed species of the order Rhizobiales. Analyses of seven cultivated Azolla species confirmed persistent association with Rhizobiales. Two distinct nearly full-length Rhizobiales genomes were identified in leaf-pocket-enriched samples from ditch grown A. filiculoides. Their annotation revealed genes for denitrification but not N-2-fixation. N-15(2) incorporation was active in ferns with N. azollae but not in ferns without. N2O was not detectably released from surface-sterilized ferns with the Rhizobiales. N-2-fixing N. azollae, we conclude, dominated the microbiome of Azolla ferns. The persistent but less abundant heterotrophic Rhizobiales bacteria possibly contributed to lowering O-2 levels in leaf pockets but did not release detectable amounts of the strong greenhouse gas N2O.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Dijkhuizen, Laura W.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Brouwer, PaulUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bolhuis, HenkUNSPECIFIEDorcid.org/0000-0002-4772-1898UNSPECIFIED
Reichart, Gert-JanUNSPECIFIEDorcid.org/0000-0002-7256-2243UNSPECIFIED
Koppers, NilsUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Huettel, BrunoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bolger, Anthony M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Li, Fay-WeiUNSPECIFIEDorcid.org/0000-0002-0076-0152UNSPECIFIED
Cheng, ShifengUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Liu, XinUNSPECIFIEDorcid.org/0000-0003-3256-2940UNSPECIFIED
Wong, Gane Ka-ShuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pryer, KathleenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Weber, AndreasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Braeutigam, AndreaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schluepmann, HenrietteUNSPECIFIEDorcid.org/0000-0001-6171-3029UNSPECIFIED
URN: urn:nbn:de:hbz:38-200884
DOI: 10.1111/nph.14843
Journal or Publication Title: New Phytol.
Volume: 217
Number: 1
Page Range: S. 453 - 467
Date: 2018
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1469-8137
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CYANOBACTERIAL SYMBIONT; MICROBIAL GENOMES; RAPID ANNOTATION; ANABAENA; IDENTIFICATION; LOCALIZATION; NITROGENASE; BACTERIA; SEQUENCEMultiple languages
Plant SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/20088

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