Universität zu Köln

Mross, Carmen, Marko, Marija, Munck, Martina, Gloeckner, Gernot, Motameny, Susanne, Altmueller, Janine, Noegel, Angelika A., Eichinger, Ludwig ORCID: 0000-0003-1594-6117, Peche, Vivek S. and Neumann, Sascha (2018). Depletion of Nesprin-2 is associated with an embryonic lethal phenotype in mice. Nucleus, 9 (1). S. 503 - 516. PHILADELPHIA: TAYLOR & FRANCIS INC. ISSN 1949-1042

Full text not available from this repository.

Abstract

Nesprin-2 is a nuclear envelope component and provides a link between cytoskeletal components of the cytoplasm and the nucleoplasm. Several isoforms are generated from its gene Syne2. Loss of the largest isoform Nesprin-2 Giant in mice is associated with a skin phenotype and altered wound healing, loss of C-terminal isoforms in mice leads to cardiomyopathies and neurological defects. Here we attempted to establish mice with an inducible knockout of all Nesprin-2 isoforms by inserting shRNA encoding sequences targeting the N- and C-terminus into the ROSA26 locus of mice. This caused early embryonic death of the animals harboring the mutant allele, which was presumably due to leaky expression of the shRNAs. Mutant embryos were only observed before E13. They had an altered appearance and were smaller in size than their wild type littermates. From this we conclude that the Nesprin-2 gene function is crucial during embryonic growth, differentiation and organogenesis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mross, Carmen UNSPECIFIED UNSPECIFIED UNSPECIFIED Marko, Marija UNSPECIFIED UNSPECIFIED UNSPECIFIED Munck, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Gloeckner, Gernot UNSPECIFIED UNSPECIFIED UNSPECIFIED Motameny, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Altmueller, Janine UNSPECIFIED UNSPECIFIED UNSPECIFIED Noegel, Angelika A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Eichinger, Ludwig UNSPECIFIED orcid.org/0000-0003-1594-6117 UNSPECIFIED Peche, Vivek S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Neumann, Sascha UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-201344
DOI:	10.1080/19491034.2018.1523664
Journal or Publication Title:	Nucleus
Volume:	9
Number:	1
Page Range:	S. 503 - 516
Date:	2018
Publisher:	TAYLOR & FRANCIS INC
Place of Publication:	PHILADELPHIA
ISSN:	1949-1042
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NUCLEAR-MEMBRANE PROTEIN; ACTIN CYTOSKELETON; ENVELOPE; DICTYOSTELIUM; FIBROBLASTS; CENTROSOME; EXPRESSION; MORPHOLOGY; ANCHORAGE; MIGRATION Multiple languages Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/20134