Universität zu Köln

Bazzi, Hisham ORCID: 0000-0001-8388-4005, Soroka, Ekaterina, Alcorn, Heather L. and Anderson, Kathryn V. (2017). STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo. Proc. Natl. Acad. Sci. U. S. A., 114 (51). S. E10928 - 9. WASHINGTON: NATL ACAD SCIENCES. ISSN 0027-8424

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Abstract

Regulated mesoderm migration is necessary for the proper morphogenesis and organ formation during embryonic development. Cell migration and its dependence on the cytoskeleton and signaling machines have been studied extensively in cultured cells; in contrast, remarkably little is known about the mechanisms that regulate mesoderm cell migration in vivo. Here, we report the identification and characterization of a mouse mutation in striatin-interacting protein 1 (Strip1) that disrupts migration of the mesoderm after the gastrulation epithelial-to-mesenchymal transition (EMT). STRIP1 is a core component of the biochemically defined mammalian striatin-interacting phosphatases and kinase (STRIPAK) complexes that appear to act through regulation of protein phosphatase 2A (PP2A), but their functions in mammals in vivo have not been examined. Strip1-null mutants arrest development at midgestation with profound disruptions in the organization of the mesoderm and its derivatives, including a complete failure of the anterior extension of axial mesoderm. Analysis of cultured mesoderm explants and mouse embryonic fibroblasts from null mutants shows that the mesoderm migration defect is correlated with decreased cell spreading, abnormal focal adhesions, changes in the organization of the actin cytoskeleton, and decreased velocity of cell migration. The results show that STRIPAK complexes are essential for cell migration and tissue morphogenesis in vivo.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Bazzi, Hisham UNSPECIFIED orcid.org/0000-0001-8388-4005 UNSPECIFIED Soroka, Ekaterina UNSPECIFIED UNSPECIFIED UNSPECIFIED Alcorn, Heather L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Anderson, Kathryn V. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-207769
DOI:	10.1073/pnas.1713535114
Journal or Publication Title:	Proc. Natl. Acad. Sci. U. S. A.
Volume:	114
Number:	51
Page Range:	S. E10928 - 9
Date:	2017
Publisher:	NATL ACAD SCIENCES
Place of Publication:	WASHINGTON
ISSN:	0027-8424
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PROTEIN PHOSPHATASE 2A; PRIMITIVE STREAK; SACCHAROMYCES-CEREVISIAE; DEVELOPMENTAL GENETICS; ENDOPLASMIC-RETICULUM; NEUROSPORA-CRASSA; CELL-MIGRATION; MICE LACKING; T-LOCUS; MORPHOGENESIS Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/20776