Universität zu Köln

Araujo, Ilka Schultheiss, Pietsch, Jessica Magdalena, Keizer, Emma Mathilde, Greese, Bettina, Balkunde, Rachappa ORCID: 0000-0002-6059-9532, Fleck, Christian and Huelskamp, Martin (2017). Stochastic gene expression in Arabidopsis thaliana. Nat. Commun., 8. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

Although plant development is highly reproducible, some stochasticity exists. This developmental stochasticity may be caused by noisy gene expression. Here we analyze the fluctuation of protein expression in Arabidopsis thaliana. Using the photoconvertible KikGR marker, we show that the protein expressions of individual cells fluctuate over time. A dual reporter system was used to study extrinsic and intrinsic noise of marker gene expression. We report that extrinsic noise is higher than intrinsic noise and that extrinsic noise in stomata is clearly lower in comparison to several other tissues/cell types. Finally, we show that cells are coupled with respect to stochastic protein expression in young leaves, hypocotyls and roots but not in mature leaves. Our data indicate that stochasticity of gene expression can vary between tissues/cell types and that it can be coupled in a non-cell-autonomous manner.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Araujo, Ilka Schultheiss UNSPECIFIED UNSPECIFIED UNSPECIFIED Pietsch, Jessica Magdalena UNSPECIFIED UNSPECIFIED UNSPECIFIED Keizer, Emma Mathilde UNSPECIFIED UNSPECIFIED UNSPECIFIED Greese, Bettina UNSPECIFIED UNSPECIFIED UNSPECIFIED Balkunde, Rachappa UNSPECIFIED orcid.org/0000-0002-6059-9532 UNSPECIFIED Fleck, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Huelskamp, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-207894
DOI:	10.1038/s41467-017-02285-7
Journal or Publication Title:	Nat. Commun.
Volume:	8
Date:	2017
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2041-1723
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SINGLE-CELL; NOISE; GROWTH; SIZE; CONSEQUENCES; LOCALIZATION; VARIABILITY; TRANSPORT; PROTEINS; FLOW Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/20789