Universität zu Köln

Heilmann, Beate, Hakkila, Kaisa ORCID: 0000-0003-3529-6895, Georg, Jens ORCID: 0000-0002-7746-5522, Tyystjarvi, Taina, Hess, Wolfgang R., Axmann, Ilka M. and Dienst, Dennis (2017). 6S RNA plays a role in recovery from nitrogen depletion in Synechocystis sp PCC 6803. BMC Microbiol., 17. LONDON: BMC. ISSN 1471-2180

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Abstract

Background: The 6S RNA is a global transcriptional riboregulator, which is exceptionally widespread among most bacterial phyla. While its role is well-characterized in some heterotrophic bacteria, we subjected a cyanobacterial homolog to functional analysis, thereby extending the scope of 6S RNA action to the special challenges of photoautotrophic lifestyles. Results: Physiological characterization of a 6S RNA deletion strain (Delta ssaA) demonstrates a delay in the recovery from nitrogen starvation. Significantly decelerated phycobilisome reassembly and glycogen degradation are accompanied with reduced photosynthetic activity compared to the wild type. Transcriptome profiling further revealed that predominantly genes encoding photosystem components, ATP synthase, phycobilisomes and ribosomal proteins were negatively affected in Delta ssaA. In vivo pull-down studies of the RNA polymerase complex indicated that the presence of 6S RNA promotes the recruitment of the cyanobacterial housekeeping s factor SigA, concurrently supporting dissociation of group 2 s factors during recovery from nitrogen starvation. Conclusions: The combination of genetic, physiological and biochemical studies reveals the homologue of 6S RNA as an integral part of the cellular response of Synechocystis sp. PCC 6803 to changing nitrogen availability. According to these results, 6S RNA supports a rapid acclimation to changing nitrogen supply by accelerating the switch from group 2 s factors SigB, SigC and SigE to SigA-dependent transcription. We therefore introduce the cyanobacterial 6S RNA as a novel candidate regulator of RNA polymerase sigma factor recruitment in Synechocystis sp. PCC 6803. Further studies on mechanistic features of the postulated interaction should shed additional light on the complexity of transcriptional regulation in cyanobacteria.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Heilmann, Beate UNSPECIFIED UNSPECIFIED UNSPECIFIED Hakkila, Kaisa UNSPECIFIED orcid.org/0000-0003-3529-6895 UNSPECIFIED Georg, Jens UNSPECIFIED orcid.org/0000-0002-7746-5522 UNSPECIFIED Tyystjarvi, Taina UNSPECIFIED UNSPECIFIED UNSPECIFIED Hess, Wolfgang R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Axmann, Ilka M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dienst, Dennis UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-208055
DOI:	10.1186/s12866-017-1137-9
Journal or Publication Title:	BMC Microbiol.
Volume:	17
Date:	2017
Publisher:	BMC
Place of Publication:	LONDON
ISSN:	1471-2180
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GROUP-2 SIGMA-FACTORS; SP STRAIN PCC-6803; STARVATION-INDUCED CHLOROSIS; LONG-TERM SURVIVAL; BACILLUS-SUBTILIS; ESCHERICHIA-COLI; NONCODING RNAS; COMPLEMENTATION ANALYSIS; GENE-EXPRESSION; CELL-SURVIVAL Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/20805