Universität zu Köln

Fleckhaus, Jan, Freire-Aradas, Ana ORCID: 0000-0002-8135-5182, Rothschild, Markus A. and Schneider, Peter M. ORCID: 0000-0003-0744-2349 (2017). Impact of genetic ancestry on chronological age prediction using DNA methylation analysis. Forensic Sci. Int. Genet. Suppl. Ser., 6. S. E399 - 2. CLARE: ELSEVIER IRELAND LTD. ISSN 1875-175X

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Abstract

Several forensic age predictors based on DNA methylation have been developed, reporting a considerable number of CpG loci highly correlated with chronological age. Most of the published age prediction models so far were based on European samples, and therefore there is a need to explore additional worldwide populations. We performed a comprehensive analysis of methylation profiles from buccal mucosa samples of three independent population groups from Middle East, West Africa and Central Europe. For each population approx. 50 samples with an equal distribution of donor ages were collected. Using bisulfite pyrosequencing we analyzed the age dependent methylation changes at five CpG sites in the genes of ASPA, ITGA2B, PDE4C and ELOVL2 and predicted the donor ages using two previously reported age prediction models. Our results display significantly lower dispersions of methylations and thus errors of predicted ages in the Middle East population compared to the Central Europe and West African populations. Interestingly, the overall methylation variation was highly similar between the three populations at all investigated CpGs.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Fleckhaus, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Freire-Aradas, Ana UNSPECIFIED orcid.org/0000-0002-8135-5182 UNSPECIFIED Rothschild, Markus A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Peter M. UNSPECIFIED orcid.org/0000-0003-0744-2349 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-208721
DOI:	10.1016/j.fsigss.2017.09.162
Journal or Publication Title:	Forensic Sci. Int. Genet. Suppl. Ser.
Volume:	6
Page Range:	S. E399 - 2
Date:	2017
Publisher:	ELSEVIER IRELAND LTD
Place of Publication:	CLARE
ISSN:	1875-175X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Genetics & Heredity Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/20872