Puchert, Malte, Pelkner, Fabian, Stein, Gregor, Angelov, Doychin N., Boltze, Johannes, Wagner, Daniel-Christoph, Odoardi, Francesca, Fluegel, Alexander, Streit, Wolfgang J. and Engele, Juergen (2017). Astrocytic expression of the CXCL12 receptor, CXCR7/ACKR3 is a hallmark of the diseased, but not developing CNS. Mol. Cell. Neurosci., 85. S. 105 - 119. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1095-9327

Full text not available from this repository.

Abstract

Based on our previous demonstration of CXCR7 as the major mediator of CXCL12 signaling in cultured astrocytes, we have now compared astrocytic expression of the CXCL12 receptors, CXCR7 and CXCR4, during CNS development and disease. In addition, we asked whether disease-associated conditions/factors affect expression of CXCL12 receptors in astrocytes. In the late embryonic rat brain, CXCR7(+)/GFAP(+) cells were restricted to the ventricular/subventricular zone while CXCR4 was widely absent from GFAP-positive cells. In the early postnatal and adult brain, CXCR7 and CXCR4 were almost exclusively expressed by GFAP-immunoreactive astrocytes forming the superficial glia limitans. Contrasting the situation in the intact CNS, a striking increase in astrocytic CXCR7 expression was detectable in the cortex of rats with experimental brain infarcts, in the spinal cord of rats with experimental autoimmune encephalomyelitis (EAE) and after mechanical compression, as well as in the in infarcted human cerebral cortex and in the hippocampus of Alzheimer's disease patients. None of these pathologies was associated with substantial increases in astrocytic CXCR4 expression. Screening of various disease-associated factors/conditions further revealed that CXCR7 expression of cultured cortical astrocytes increases with IFN gamma as well as under hypoxic conditions whereas CXCR7 expression is attenuated following treatment with IFN beta. Again, none of the treatments affected CXCR4 expression in cultured astrocytes. Together, these findings support the hypothesis of a crucial role of astrocytic CXCR7 in the progression of various CNS pathologies.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Puchert, MalteUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pelkner, FabianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stein, GregorUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Angelov, Doychin N.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Boltze, JohannesUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wagner, Daniel-ChristophUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Odoardi, FrancescaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fluegel, AlexanderUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Streit, Wolfgang J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Engele, JuergenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-209240
DOI: 10.1016/j.mcn.2017.09.001
Journal or Publication Title: Mol. Cell. Neurosci.
Volume: 85
Page Range: S. 105 - 119
Date: 2017
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication: SAN DIEGO
ISSN: 1095-9327
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
TRAUMATIC BRAIN-INJURY; CENTRAL-NERVOUS-SYSTEM; EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; IMPROVES FUNCTIONAL RECOVERY; NEURAL PROGENITOR CELLS; EPIDERMAL-GROWTH-FACTOR; SPINAL-CORD-INJURY; HUMAN GLIOMA-CELLS; CHEMOKINE RECEPTOR; REACTIVE ASTROGLIOSISMultiple languages
NeurosciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/20924

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item