Universität zu Köln

Mackmull, Marie-Therese ORCID: 0000-0003-2928-1144, Klaus, Bernd, Heinze, Ivonne, Chokkalingam, Manopriya, Beyer, Andreas ORCID: 0000-0002-3891-2123, Russell, Robert B., Ori, Alessandro ORCID: 0000-0002-3046-0871 and Beck, Martin ORCID: 0000-0002-7397-1321 (2017). Landscape of nuclear transport receptor cargo specificity. Mol. Syst. Biol., 13 (12). HOBOKEN: WILEY. ISSN 1744-4292

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Abstract

Nuclear transport receptors (NTRs) recognize localization signals of cargos to facilitate their passage across the central channel of nuclear pore complexes (NPCs). About 30 different NTRs constitute different transport pathways in humans and bind to a multitude of different cargos. The exact cargo spectrum of the majority of NTRs, their specificity and even the extent to which active nucleocytoplasmic transport contributes to protein localization remains understudied because of the transient nature of these interactions and the wide dynamic range of cargo concentrations. To systematically map cargo-NTR relationships in situ, we used proximity ligation coupled to mass spectrometry (BioID). We systematically fused the engineered biotin ligase BirA* to 16 NTRs. We estimate that a considerable fraction of the human proteome is subject to active nuclear transport. We quantified the specificity and redundancy in NTR interactions and identified transport pathways for cargos. We extended the BioID method by the direct identification of biotinylation sites. This approach enabled us to identify interaction interfaces and to discriminate direct versus piggyback transport mechanisms. Data are available via ProteomeXchange with identifier PXD007976.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mackmull, Marie-Therese UNSPECIFIED orcid.org/0000-0003-2928-1144 UNSPECIFIED Klaus, Bernd UNSPECIFIED UNSPECIFIED UNSPECIFIED Heinze, Ivonne UNSPECIFIED UNSPECIFIED UNSPECIFIED Chokkalingam, Manopriya UNSPECIFIED UNSPECIFIED UNSPECIFIED Beyer, Andreas UNSPECIFIED orcid.org/0000-0002-3891-2123 UNSPECIFIED Russell, Robert B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ori, Alessandro UNSPECIFIED orcid.org/0000-0002-3046-0871 UNSPECIFIED Beck, Martin UNSPECIFIED orcid.org/0000-0002-7397-1321 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-210304
DOI:	10.15252/msb.20177608
Journal or Publication Title:	Mol. Syst. Biol.
Volume:	13
Number:	12
Date:	2017
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1744-4292
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IMPORTIN-ALPHA; NUCLEOCYTOPLASMIC TRANSPORT; MASS-SPECTROMETRY; GENE-EXPRESSION; PORE COMPLEXES; MAMMALIAN-CELLS; AMINO-ACIDS; PROTEIN; IDENTIFICATION; EXPORT Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21030