Universität zu Köln

Moejes, Fiona Wanjiku ORCID: 0000-0002-7464-7590, Succurro, Antonella ORCID: 0000-0003-0227-2803, Popa, Ovidiu, Maguire, Julie and Ebenhoeh, Oliver ORCID: 0000-0002-7229-7398 (2017). Dynamics of the Bacterial Community Associated with Phaeodactylum tricornutum Cultures. Processes, 5 (4). BASEL: MDPI AG. ISSN 2227-9717

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Abstract

The pennate diatom Phaeodactylum tricornutum is a model organism able to synthesize industrially-relevant molecules. Commercial-scale cultivation currently requires large monocultures, prone to bio-contamination. However, little is known about the identity of the invading organisms. To reduce the complexity of natural systems, we systematically investigated the microbiome of non-axenic P. tricornutum cultures from a culture collection in reproducible experiments. The results revealed a dynamic bacterial community that developed differently in complete and minimal media conditions. In complete media, we observed an accelerated culture crash, indicating a more stable culture in minimal media. The identification of only four bacterial families as major players within the microbiome suggests specific roles depending on environmental conditions. From our results we propose a network of putative interactions between P. tricornutum and these main bacterial factions. We demonstrate that, even with rather sparse data, a mathematical model can be reconstructed that qualitatively reproduces the observed population dynamics, thus indicating that our hypotheses regarding the molecular interactions are in agreement with experimental data. Whereas the model in its current state is only qualitative, we argue that it serves as a starting point to develop quantitative and predictive mathematical models, which may guide experimental efforts to synthetically construct and monitor stable communities required for robust upscaling strategies.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Moejes, Fiona Wanjiku UNSPECIFIED orcid.org/0000-0002-7464-7590 UNSPECIFIED Succurro, Antonella UNSPECIFIED orcid.org/0000-0003-0227-2803 UNSPECIFIED Popa, Ovidiu UNSPECIFIED UNSPECIFIED UNSPECIFIED Maguire, Julie UNSPECIFIED UNSPECIFIED UNSPECIFIED Ebenhoeh, Oliver UNSPECIFIED orcid.org/0000-0002-7229-7398 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-210432
DOI:	10.3390/pr5040077
Journal or Publication Title:	Processes
Volume:	5
Number:	4
Date:	2017
Publisher:	MDPI AG
Place of Publication:	BASEL
ISSN:	2227-9717
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MICROBIAL COMMUNITIES; MARINE PROTEOBACTERIA; TRACE-METALS; FATTY-ACID; FAM. NOV.; PSEUDOALTEROMONAS; GROWTH; ALGAE; PHYTOPLANKTON; ALTEROMONAS Multiple languages Engineering, Chemical Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21043