Uribe, P., Villegas, J. V., Boguen, R., Treulen, F., Sanchez, R., Mallmann, P., Isachenko, V., Rahimi, G. and Isachenko, E. (2017). Use of the fluorescent dye tetramethylrhodamine methyl ester perchlorate for mitochondrial membrane potential assessment in human spermatozoa. Andrologia, 49 (9). HOBOKEN: WILEY. ISSN 1439-0272

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Abstract

Mitochondrial membrane potential (Delta psi m) is an indicator of sperm quality and its evaluation complements the standard semen analysis. The fluorescent dye JC-1 has been widely used to assess sperm Delta psi m; however, some problems have been detected under certain experimental conditions. Another fluorescent compound, tetramethylrhodamine methyl ester perchlorate (TMRM), has been used in somatic cells and bovine spermatozoa but not in human spermatozoa. TMRM accumulates in hyperpolarised mitochondria and the fluorescence intensity of this compound correlates with Delta psi m. Thus, the aim of this study was to evaluate and validate the usefulness of the fluorescent dye TMRM for measuring sperm Delta psi m. The results showed that TMRM accurately detects sperm populations displaying either high or low Delta psi m. Moreover, TMRM was able to measure sperm Delta psi m under the experimental conditions in which JC-1 had previously presented difficulties. Differences in Delta psi m according to sperm and semen quality were properly detected and a positive correlation between Delta psi m and conventional semen parameters was observed. Finally, a positive correlation was found between the Delta psi m measurement by TMRM and by the widely used JC-1. In conclusion, TMRM is a simple, time-effective method, easy to set in laboratories equipped with flow cytometry technology, and can accurately detect changes in Delta psi m with efficiency comparable to JC-1 without its limitations.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Uribe, P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Villegas, J. V.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Boguen, R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Treulen, F.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sanchez, R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mallmann, P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Isachenko, V.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rahimi, G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Isachenko, E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-212662
DOI: 10.1111/and.12753
Journal or Publication Title: Andrologia
Volume: 49
Number: 9
Date: 2017
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1439-0272
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
TOTAL ANTIOXIDANT CAPACITY; OXIDATIVE STRESS; SEMINAL PLASMA; MALE-INFERTILITY; OXYGEN; MEN; SEMEN; SERUM; COENZYME-Q10; ASSOCIATIONMultiple languages
AndrologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/21266

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