Universität zu Köln

El-Kashef, Noha, Gomes, Iva ORCID: 0000-0002-1593-301X, Mercer-Chalmers-Bender, Katja ORCID: 0000-0002-3843-9065, Schneider, Peter M. ORCID: 0000-0003-0744-2349, Rothschild, Markus A. and Juebner, Martin (2017). Comparative proteome analysis for identification of differentially abundant proteins in SIDS. Int. J. Legal Med., 131 (6). S. 1597 - 1614. NEW YORK: SPRINGER. ISSN 1437-1596

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Abstract

Sudden infant death syndrome (SIDS) remains one of the most common causes of post-neonatal infant mortality in developed countries. Its pathogenesis is still poorly understood. The goal of the present study was to characterize changes in the proteome of SIDS compared to age-matched controls in heart and medulla tissues as well as in blood samples using two complementary quantitative proteomic techniques: 2D-DIGE and iTRAQ aiming to provide new insights into the mechanism of SIDS and to find diagnostic protein patterns. Our results revealed collectively 122 modulated proteins in SIDS of which 83 proteins were up-regulated. They are involved in metabolic processes, cellular processes, and localization. Gene expression patterns of selected proteins were further validated by reverse transcription quantitative real-time PCR (RT-qPCR). The role of hypoxia, inflammation, and apoptosis in SIDS was demonstrated by exploring some candidate proteins especially APOA1, GAPDH, S100B, zyxin, and complement component C4A. According to the results of this study, these proteins might be used as diagnostic biomarkers for SIDS. All of them were up-regulated in SIDS except for C4A that was down-regulated.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code El-Kashef, Noha UNSPECIFIED UNSPECIFIED UNSPECIFIED Gomes, Iva UNSPECIFIED orcid.org/0000-0002-1593-301X UNSPECIFIED Mercer-Chalmers-Bender, Katja UNSPECIFIED orcid.org/0000-0002-3843-9065 UNSPECIFIED Schneider, Peter M. UNSPECIFIED orcid.org/0000-0003-0744-2349 UNSPECIFIED Rothschild, Markus A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Juebner, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-212855
DOI:	10.1007/s00414-017-1632-4
Journal or Publication Title:	Int. J. Legal Med.
Volume:	131
Number:	6
Page Range:	S. 1597 - 1614
Date:	2017
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1437-1596
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SUDDEN-INFANT-DEATH; FETAL-HEMOGLOBIN LEVELS; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; CEREBROSPINAL-FLUID; GEL-ELECTROPHORESIS; GENE-EXPRESSION; S100B PROTEIN; ISOBARIC TAGS; APOPTOSIS; HYPOXIA Multiple languages Medicine, Legal Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21285