Universität zu Köln

Hujer, Andrea M., Higgins, Paul G. ORCID: 0000-0001-8677-9454, Rudin, Susan D., Buser, Genevieve L., Marshall, Steven H., Xanthopoulou, Kyriaki ORCID: 0000-0001-8591-8184, Seifert, Harald, Rojas, Laura J., Domitrovic, T. Nicholas, Cassidy, P. Maureen, Cunningham, Margaret C., Vega, Robert, Furuno, Jon P., Pfeiffer, Christopher D., Beldavs, Zintars G., Wright, Meredith S., Jacobs, Michael R., Adams, Mark D. and Bonomo, Robert A. (2017). Nosocomial Outbreak of Extensively Drug-Resistant Acinetobacter baumannii Isolates Containing bla(OXA-237) Carried on a Plasmid. Antimicrob. Agents Chemother., 61 (11). WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1098-6596

Full text not available from this repository.

Abstract

Carbapenem antibiotics are among the mainstays for treating infections caused by Acinetobacter baumannii, especially in the Northwest United States, where carbapenem-resistant A. baumannii remains relatively rare. However, between June 2012 and October 2014, an outbreak of carbapenem-resistant A. baumannii occurred in 16 patients from five health care facilities in the state of Oregon. All isolates were defined as extensively drug resistant. Multilocus sequence typing revealed that the isolates belonged to sequence type 2 (international clone 2 [IC2]) and were > 95% similar as determined by repetitive-sequence-based PCR analysis. Multiplex PCR revealed the presence of a bla(OXA) carbapenemase gene, later identified as bla(OXA-237). Whole-genome sequencing of all isolates revealed a well-supported separate branch within a global A. baumannii phylogeny. Pacific Biosciences (PacBio) SMRT sequencing was also performed on one isolate to gain insight into the genetic location of the carbapenem resistance gene. We discovered that blaOXA-237, flanked on either side by ISAba1 elements in opposite orientations, was carried on a 15,198-bp plasmid designated pORAB01-3 and was present in all 16 isolates. The plasmid also contained genes encoding a TonB-dependent receptor, septicolysin, a type IV secretory pathway (VirD4 component, TraG/TraD family) ATPase, an integrase, a RepB family plasmid DNA replication initiator protein, an alpha/beta hydrolase, and a BrnT/BrnA type II toxin-antitoxin system. This is the first reported outbreak in the northwestern United States associated with this carbapenemase. Particularly worrisome is that blaOXA-237 was carried on a plasmid and found in the most prominent worldwide clonal group IC2, potentially giving pORAB01-3 great capacity for future widespread dissemination.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hujer, Andrea M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED Rudin, Susan D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Buser, Genevieve L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Marshall, Steven H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Xanthopoulou, Kyriaki UNSPECIFIED orcid.org/0000-0001-8591-8184 UNSPECIFIED Seifert, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED Rojas, Laura J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Domitrovic, T. Nicholas UNSPECIFIED UNSPECIFIED UNSPECIFIED Cassidy, P. Maureen UNSPECIFIED UNSPECIFIED UNSPECIFIED Cunningham, Margaret C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Vega, Robert UNSPECIFIED UNSPECIFIED UNSPECIFIED Furuno, Jon P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfeiffer, Christopher D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Beldavs, Zintars G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wright, Meredith S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Jacobs, Michael R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Adams, Mark D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bonomo, Robert A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-213275
DOI:	10.1128/AAC.00797-17
Journal or Publication Title:	Antimicrob. Agents Chemother.
Volume:	61
Number:	11
Date:	2017
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	1098-6596
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PREVALENT OXA CARBAPENEMASES; ACUTE-CARE FACILITIES; IV SECRETION SYSTEM; KLEBSIELLA-PNEUMONIAE; GENES; VIRULENCE; PCR; EPIDEMIOLOGY; CONJUGATION; ORGANISMS Multiple languages Microbiology; Pharmacology & Pharmacy Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21327