Universität zu Köln

Kraus, Christopher, Schiffer, Philipp H., Kagoshima, Hiroshi, Hiraki, Hideaki, Vogt, Theresa, Kroiher, Michael, Kohara, Yuji and Schierenberg, Einhard (2017). Differences in the genetic control of early egg development and reproduction between C-elegans and its parthenogenetic relative D-coronatus. EvoDevo, 8. LONDON: BMC. ISSN 2041-9139

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Abstract

Background: The free-living nematode Diploscapter coronatus is the closest known relative of Caenorhabditis elegans with parthenogenetic reproduction. It shows several developmental idiosyncracies, for example concerning the mode of reproduction, embryonic axis formation and early cleavage pattern (Lahl et al. in Int J Dev Biol 50:393-397, 2006). Our recent genome analysis (Hiraki et al. in BMC Genomics 18:478, 2017) provides a solid foundation to better understand the molecular basis of developmental idiosyncrasies in this species in an evolutionary context by comparison with selected other nematodes. Our genomic data also yielded indications for the view that D. coronatus is a product of interspecies hybridization. Results: In a genomic comparison between D. coronatus, C. elegans, other representatives of the genus Caenorhabditis and the more distantly related Pristionchus pacificus and Panagrellus redivivus, certain genes required for central developmental processes in C. elegans like control of meiosis and establishment of embryonic polarity were found to be restricted to the genus Caenorhabditis. The mRNA content of early D. coronatus embryos was sequenced and compared with similar stages in C. elegans and Ascaris suum. We identified 350 gene families transcribed in the early embryo of D. coronatus but not in the other two nematodes. Looking at individual genes transcribed early in D. coronatus but not in C. elegans and A. suum, we found that orthologs of most of these are present in the genomes of the latter species as well, suggesting heterochronic shifts with respect to expression behavior. Considerable genomic heterozygosity and allelic divergence lend further support to the view that D. coronatus may be the result of an interspecies hybridization. Expression analysis of early acting single-copy genes yields no indication for silencing of one parental genome. Conclusions: Our comparative cellular and molecular studies support the view that the genus Caenorhabditis differs considerably from the other studied nematodes in its control of development and reproduction. The easy-to-culture parthenogenetic D. coronatus, with its high-quality draft genome and only a single chromosome when haploid, offers many new starting points on the cellular, molecular and genomic level to explore alternative routes of nematode development and reproduction.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kraus, Christopher UNSPECIFIED UNSPECIFIED UNSPECIFIED Schiffer, Philipp H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kagoshima, Hiroshi UNSPECIFIED UNSPECIFIED UNSPECIFIED Hiraki, Hideaki UNSPECIFIED UNSPECIFIED UNSPECIFIED Vogt, Theresa UNSPECIFIED UNSPECIFIED UNSPECIFIED Kroiher, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Kohara, Yuji UNSPECIFIED UNSPECIFIED UNSPECIFIED Schierenberg, Einhard UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-214112
DOI:	10.1186/s13227-017-0081-y
Journal or Publication Title:	EvoDevo
Volume:	8
Date:	2017
Publisher:	BMC
Place of Publication:	LONDON
ISSN:	2041-9139
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language OOCYTE MEIOTIC MATURATION; LETHAL GIANT LARVAE; CAENORHABDITIS-ELEGANS; PHYLOGENETIC-RELATIONSHIPS; SPINDLE ORIENTATION; PATTERN-FORMATION; SYSTEM DRIFT; DRAFT GENOME; SEQUENCE; PROTEIN Multiple languages Evolutionary Biology; Developmental Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21411