Universität zu Köln

Schacherl, Magdalena ORCID: 0000-0002-5478-2509, Gompert, Monika, Pardon, Els, Lamkemeyer, Tobias, Steyaert, Jan ORCID: 0000-0002-3825-874X and Baumann, Ulrich (2017). Crystallographic and biochemical characterization of the dimeric architecture of site-2 protease. Biochim. Biophys. Acta-Biomembr., 1859 (10). S. 1859 - 1872. AMSTERDAM: ELSEVIER. ISSN 1879-2642

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Abstract

Regulated intramembrane proteolysis by members of the site-2 protease family (S2P) is an essential signal transduction mechanism conserved from bacteria to humans. There is some evidence that extra-membranous domains, like PDZ and CBS domains, regulate the proteolytic activity of S2Ps and that some members act as dimers. Here we report the crystal structure of the regulatory CBS domain pair of S2P from Archaeoglobus fulgidus, AJS2P, in the apo and nucleotide-bound form in complex with a specific nanobody from llama. Cross-linking and SEC-MALS analyses show for the first time the dimeric architecture of AlS2P both in the membrane and in detergent micelles. The CBS domain pair dimer (CBS module) displays an unusual head-to-tail configuration and nucleotide binding triggers no major conformational changes in the magnesium-free state. In solution, MgATP drives monomerization of the CBS module. We propose a model of the so far unknown architecture of the transmembrane domain dimer and for a regulatory mechanism of AfS2P that involves the interaction of positively charged arginine residues located at the cytoplasmic face of the transmembrane domain with the negatively charged phosphate groups of ATP moieties bound to the CBS domain pairs. Binding of MgATP could promote opening of the CBS module to allow lateral access of the globular cytoplasmic part of the substrate. (C) 2017 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schacherl, Magdalena UNSPECIFIED orcid.org/0000-0002-5478-2509 UNSPECIFIED Gompert, Monika UNSPECIFIED UNSPECIFIED UNSPECIFIED Pardon, Els UNSPECIFIED UNSPECIFIED UNSPECIFIED Lamkemeyer, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Steyaert, Jan UNSPECIFIED orcid.org/0000-0002-3825-874X UNSPECIFIED Baumann, Ulrich UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-216393
DOI:	10.1016/j.bbamem.2017.05.006
Journal or Publication Title:	Biochim. Biophys. Acta-Biomembr.
Volume:	1859
Number:	10
Page Range:	S. 1859 - 1872
Date:	2017
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1879-2642
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ESCHERICHIA-COLI; CBS DOMAINS; MEMBRANE TOPOLOGY; CRYSTAL-STRUCTURE; BINDING; CLEAVAGE; SUBSTRATE; CELL; ATP; RECOGNITION Multiple languages Biochemistry & Molecular Biology; Biophysics Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21639