Universität zu Köln

Hinkelbein, Jochen, Jansen, Stefanie, Iovino, Ivan, Kruse, Sylvia, Meyer, Moritz, Cirillo, Fabrizio, Drinhaus, Hendrik, Hohn, Andreas, Klein, Corinna, De Robertis, Edoardo ORCID: 0000-0001-9182-6964 and Beutner, Dirk (2017). Thirty Minutes of Hypobaric Hypoxia Provokes Alterations of Immune Response, Haemostasis, and Metabolism Proteins in Human Serum. Int. J. Mol. Sci., 18 (9). BASEL: MDPI. ISSN 1422-0067

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Abstract

Hypobaric hypoxia (HH) during airline travel induces several (patho-) physiological reactions in the human body. Whereas severe hypoxia is investigated thoroughly, very little is known about effects of moderate or short-term hypoxia, e.g. during airline flights. The aim of the present study was to analyse changes in serum protein expression and activation of signalling cascades in human volunteers staying for 30 min in a simulated altitude equivalent to airline travel. After approval of the local ethics committee, 10 participants were exposed to moderate hypoxia (simulation of 2400 m or 8000 ft for 30 min) in a hypobaric pressure chamber. Before and after hypobaric hypoxia, serum was drawn, centrifuged, and analysed by two-dimensional gel electrophoresis (2-DIGE) and matrix-assisted laser desorption/ionization followed by time-of-flight mass spectrometry (MALDI-TOF). Biological functions of regulated proteins were identified using functional network analysis (GeneMania((R)), STRING((R)), and Perseus((R)) software). In participants, oxygen saturation decreased from 98.1 +/- 1.3% to 89.2 +/- 1.8% during HH. Expression of 14 spots (i.e., 10 proteins: ALB, PGK1, APOE, GAPDH, C1QA, C1QB, CAT, CA1, F2, and CLU) was significantly altered. Bioinformatic analysis revealed an association of the altered proteins with the signalling cascades regulation of haemostasis (four proteins), metabolism (five proteins), and leukocyte mediated immune response (five proteins). Even though hypobaric hypoxia was short and moderate (comparable to an airliner flight), analysis of protein expression in human subjects revealed an association to immune response, protein metabolism, and haemostasis

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hinkelbein, Jochen UNSPECIFIED UNSPECIFIED UNSPECIFIED Jansen, Stefanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Iovino, Ivan UNSPECIFIED UNSPECIFIED UNSPECIFIED Kruse, Sylvia UNSPECIFIED UNSPECIFIED UNSPECIFIED Meyer, Moritz UNSPECIFIED UNSPECIFIED UNSPECIFIED Cirillo, Fabrizio UNSPECIFIED UNSPECIFIED UNSPECIFIED Drinhaus, Hendrik UNSPECIFIED UNSPECIFIED UNSPECIFIED Hohn, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Klein, Corinna UNSPECIFIED UNSPECIFIED UNSPECIFIED De Robertis, Edoardo UNSPECIFIED orcid.org/0000-0001-9182-6964 UNSPECIFIED Beutner, Dirk UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-218484
DOI:	10.3390/ijms18091882
Journal or Publication Title:	Int. J. Mol. Sci.
Volume:	18
Number:	9
Date:	2017
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	1422-0067
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language 2-DIMENSIONAL ELECTROPHORESIS; COMPUTATIONAL PLATFORM; EUSTACHIAN-TUBE; EXPRESSION; COAGULATION; ADAPTATION; ACTIVATION; TRANSCRIPTION; ASTROCYTES; PROTEOMICS Multiple languages Biochemistry & Molecular Biology; Chemistry, Multidisciplinary Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21848