Goeckeritz, Elisa, Vondey, Verena, Guastafierro, Anna, Pizevska, Maja, Hassenrueck, Floyd, Neumann, Lars, Hallek, Michael and Krause, Guenter (2017). Establishing a chemical genetic link between Bruton tyrosine kinase activity in malignant B cells and cell functions involved in the micro-environmental dialogue. Br. J. Haematol., 178 (6). S. 949 - 954. HOBOKEN: WILEY. ISSN 1365-2141
Full text not available from this repository.Abstract
To elucidate their mechanism of action, inhibitors of Bruton tyrosine kinase (BTK) and resistant BTK mutants were employed to dissect target-dependent cellular functions. BTK-C481S and -T474I, expressed in Ramos and NALM-6 cells, maintained BTK auto-phosphorylation under treatment with ibrutinib or dasatinib, respectively, which showed only modest cytotoxicity. Retained activity of BTK-T474 partially rescued cell migration from inhibition by dasatinib. Importantly, resistant BTK mutants reconstituted B cell receptor-triggered chemokine secretion in the presence of corresponding inhibitors, demonstrating that BTK activity is connected with cell-intrinsic functions of malignant B cells with importance for their dialogue with the micro-environment.
| Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||
| Creators: |
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| URN: | urn:nbn:de:hbz:38-219345 | ||||||||||||||||||||||||||||||||||||
| DOI: | 10.1111/bjh.14781 | ||||||||||||||||||||||||||||||||||||
| Journal or Publication Title: | Br. J. Haematol. | ||||||||||||||||||||||||||||||||||||
| Volume: | 178 | ||||||||||||||||||||||||||||||||||||
| Number: | 6 | ||||||||||||||||||||||||||||||||||||
| Page Range: | S. 949 - 954 | ||||||||||||||||||||||||||||||||||||
| Date: | 2017 | ||||||||||||||||||||||||||||||||||||
| Publisher: | WILEY | ||||||||||||||||||||||||||||||||||||
| Place of Publication: | HOBOKEN | ||||||||||||||||||||||||||||||||||||
| ISSN: | 1365-2141 | ||||||||||||||||||||||||||||||||||||
| Language: | English | ||||||||||||||||||||||||||||||||||||
| Faculty: | Unspecified | ||||||||||||||||||||||||||||||||||||
| Divisions: | Unspecified | ||||||||||||||||||||||||||||||||||||
| Subjects: | no entry | ||||||||||||||||||||||||||||||||||||
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| Refereed: | Yes | ||||||||||||||||||||||||||||||||||||
| URI: | http://kups.ub.uni-koeln.de/id/eprint/21934 |
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