Universität zu Köln

Sutipatanasomboon, Arpaporn ORCID: 0000-0001-7642-5310, Herberth, Stefanie, Alwood, Ellen G., Haeweker, Heidrun, Mueller, Britta, Shahriari, Mojgan, Zienert, Anke Y., Marin, Birger, Robatzek, Silke, Praefcke, Gerrit J. K., Ayscough, Kathryn R., Huelskamp, Martin and Schellmann, Swen (2017). Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death. Sci Rep, 7. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

Cell death, autophagy and endosomal sorting contribute to many physiological, developmental and immunological processes in plants. They are mechanistically interconnected and interdependent, but the molecular basis of their mutual regulation has only begun to emerge in plants. Here, we describe the identification and molecular characterization of CELL DEATH RELATED ENDOSOMAL FYVE/SYLF PROTEIN 1 (CFS1). The CFS1 protein interacts with the ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT I (ESCRT-I) component ELCH (ELC) and is localized at ESCRT-I-positive late endosomes likely through its PI3P and actin binding SH3YL1 Ysc84/Lsb4p Lsb3p plant FYVE (SYLF) domain. Mutant alleles of cfs1 exhibit auto-immune phenotypes including spontaneous lesions that show characteristics of hypersensitive response (HR). Autoimmunity in cfs1 is dependent on ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)-mediated effector-triggered immunity (ETI) but independent from salicylic acid. Additionally, cfs1 mutants accumulate the autophagy markers ATG8 and NBR1 independently from EDS1. We hypothesize that CFS1 acts at the intersection of autophagosomes and endosomes and contributes to cellular homeostasis by mediating autophagosome turnover.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sutipatanasomboon, Arpaporn UNSPECIFIED orcid.org/0000-0001-7642-5310 UNSPECIFIED Herberth, Stefanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Alwood, Ellen G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Haeweker, Heidrun UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Britta UNSPECIFIED UNSPECIFIED UNSPECIFIED Shahriari, Mojgan UNSPECIFIED UNSPECIFIED UNSPECIFIED Zienert, Anke Y. UNSPECIFIED UNSPECIFIED UNSPECIFIED Marin, Birger UNSPECIFIED UNSPECIFIED UNSPECIFIED Robatzek, Silke UNSPECIFIED UNSPECIFIED UNSPECIFIED Praefcke, Gerrit J. K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ayscough, Kathryn R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Huelskamp, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Schellmann, Swen UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-221709
DOI:	10.1038/s41598-017-08577-8
Journal or Publication Title:	Sci Rep
Volume:	7
Date:	2017
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SALICYLIC-ACID; ESCRT COMPONENT; SIGNALING PATHWAYS; DISEASE RESISTANCE; IMMUNE-RESPONSE; ARABIDOPSIS; MUTANTS; SENESCENCE; INDUCTION; MACHINERY Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22170