Universität zu Köln

Shydlovskyi, Sergii, Zienert, Anke Y., Ince, Semra, Dovengerds, Christine, Hohendahl, Annika, Dargazanli, Julia M., Blum, Ailisa ORCID: 0000-0003-4666-9255, Guenther, Saskia D., Kladt, Nikolay, Stuerzl, Michael, Schauss, Astrid C., Kutsch, Miriam ORCID: 0000-0002-9346-2196, Roux, Aurelien, Praefcke, Gerrit J. K. and Herrmann, Christian (2017). Nucleotide-dependent farnesyl switch orchestrates polymerization and membrane binding of human guanylate-binding protein 1. Proc. Natl. Acad. Sci. U. S. A., 114 (28). S. E5559 - 10. WASHINGTON: NATL ACAD SCIENCES. ISSN 0027-8424

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Abstract

Dynamin-like proteins (DLPs) mediate various membrane fusion and fission processes within the cell, which often require the polymerization of DLPs. An IFN-inducible family of DLPs, the guanylate-binding proteins (GBPs), is involved in antimicrobial and antiviral responses within the cell. Human guanylate-binding protein 1 (hGBP1), the founding member of GBPs, is also engaged in the regulation of cell adhesion and migration. Here, we show how the GTPase cycle of farnesylated hGBP1 (hGBP1F) regulates its self-assembly and membrane interaction. Using vesicles of various sizes as a lipid bilayer model, we show GTP-dependent membrane binding of hGBP1F. In addition, we demonstrate nucleotide-dependent tethering ability of hGBP1F. Furthermore, we report nucleotide-dependent polymerization of hGBP1F, which competes with membrane binding of the protein. Our results show that hGBP1F acts as a nucleotide-controlled molecular switch by modulating the accessibility of its farnesyl moiety, which does not require any supportive proteins.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Shydlovskyi, Sergii UNSPECIFIED UNSPECIFIED UNSPECIFIED Zienert, Anke Y. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ince, Semra UNSPECIFIED UNSPECIFIED UNSPECIFIED Dovengerds, Christine UNSPECIFIED UNSPECIFIED UNSPECIFIED Hohendahl, Annika UNSPECIFIED UNSPECIFIED UNSPECIFIED Dargazanli, Julia M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Blum, Ailisa UNSPECIFIED orcid.org/0000-0003-4666-9255 UNSPECIFIED Guenther, Saskia D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kladt, Nikolay UNSPECIFIED UNSPECIFIED UNSPECIFIED Stuerzl, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Schauss, Astrid C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kutsch, Miriam UNSPECIFIED orcid.org/0000-0002-9346-2196 UNSPECIFIED Roux, Aurelien UNSPECIFIED UNSPECIFIED UNSPECIFIED Praefcke, Gerrit J. K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Herrmann, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-225039
DOI:	10.1073/pnas.1620959114
Journal or Publication Title:	Proc. Natl. Acad. Sci. U. S. A.
Volume:	114
Number:	28
Page Range:	S. E5559 - 10
Date:	2017
Publisher:	NATL ACAD SCIENCES
Place of Publication:	WASHINGTON
ISSN:	0027-8424
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INTERFERON-INDUCIBLE GTPASES; GTP HYDROLYSIS; DYNAMIN SUPERFAMILY; ANTIVIRAL ACTIVITY; GAMMA; HGBP1; FUSION; MACROPHAGES; FISSION; GMP Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22503