Universität zu Köln

Hahn, Florian ORCID: 0000-0002-0215-3209, Eisenhut, Marion, Mantegazza, Otho and Weber, Andreas P. M. (2017). Generation of Targeted Knockout Mutants in Arabidopsis thaliana Using CRISPR/Cas9. Bio-protocol, 7 (13). SUNNYVALE: BIO-PROTOCOL. ISSN 2331-8325

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Abstract

The CRISPR/Cas9 system has emerged as a powerful tool for gene editing in plants and beyond. We have developed a plant vector system for targeted Cas9-dependent mutagenesis of genes in up to two different target sites in Arabidopsis thaliana. This protocol describes a simple 1-week cloning procedure for a single T-DNA vector containing the genes for Cas9 and sgRNAs, as well as the detection of induced mutations in planta. The procedure can likely be adapted for other transformable plant species.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hahn, Florian UNSPECIFIED orcid.org/0000-0002-0215-3209 UNSPECIFIED Eisenhut, Marion UNSPECIFIED UNSPECIFIED UNSPECIFIED Mantegazza, Otho UNSPECIFIED UNSPECIFIED UNSPECIFIED Weber, Andreas P. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-225139
DOI:	10.21769/BioProtoc.2384
Journal or Publication Title:	Bio-protocol
Volume:	7
Number:	13
Date:	2017
Publisher:	BIO-PROTOCOL
Place of Publication:	SUNNYVALE
ISSN:	2331-8325
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22513