Merino, O., Figueroa, E., Cheuqueman, C., Valdebenito, I., Isachenko, V., Isachenko, E., Sanchez, R., Farias, J. and Risopatron, J. (2017). Short-term storage of salmonids semen in a sodium alginate-based extender. Andrologia, 49 (5). HOBOKEN: WILEY. ISSN 1439-0272

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Abstract

Short-term storage of semen is a useful strategy for preservation of fish spermatozoa. However, there is a significantly decrease on sperm function mainly due to oxidative stress. In this way, sodium alginate plays an important role as free radical scavenger compound. Accordingly, the aim of our study was to analyse the effect of a sodium alginate-based extender on sperm function in the short-term storage of salmonids semen. Samples of Salmo salar, Oncorhynchus kisutch, and Oncorhynchus mykiss were stored in Storfish((R)) (Ext-C) and Storfish((R)) supplemented with sodium alginate (Ext-A) during 10days at 4 degrees C. After storage, motility, viability, mitochondrial membrane potential (mit), superoxide anion (O-2(-)) level and DNA fragmentation (DNA Frag) were assessed. Ext-A had positive effect in preservation of sperm motility, viability, mit, O-2(-) level and DNA integrity in the three species analysed compared to control samples. In Ext-A, the spermatozoa of S.salar and O.mykiss showed significantly higher motility, viability and mit than O.kisutch. However, O.kisutch and O.mykiss had significantly lower O-2(-) level than S.salar, and DNA fragmentation in O.kisutch and S.salar was significantly lower than in samples of O.mykiss (p<0.05). Dilution of salmonids semen in a sodium alginate-based extender is effective for protecting sperm quality during 10days of short-term storage.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Merino, O.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Figueroa, E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cheuqueman, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Valdebenito, I.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Isachenko, V.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Isachenko, E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sanchez, R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Farias, J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Risopatron, J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-230270
DOI: 10.1111/and.12661
Journal or Publication Title: Andrologia
Volume: 49
Number: 5
Date: 2017
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1439-0272
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
TROUT ONCORHYNCHUS-MYKISS; REVERSED RAINBOW-TROUT; OXIDATIVE STRESS; DNA FRAGMENTATION; SUPEROXIDE ANION; SEMINAL PLASMA; SPERM; QUALITY; SPERMATOZOA; PARAMETERSMultiple languages
AndrologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/23027

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