Universität zu Köln

Mitosch, Karin, Rieckh, Georg and Bollenbach, Tobias ORCID: 0000-0003-4398-476X (2017). Noisy Response to Antibiotic Stress Predicts Subsequent Single-Cell Survival in an Acidic Environment. Cell Syst., 4 (4). S. 393 - 409. CAMBRIDGE: CELL PRESS. ISSN 2405-4720

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Abstract

Antibiotics elicit drastic changes in microbial gene expression, including the induction of stress response genes. While certain stress responses are known to cross-protect bacteria fromother stressors, it is unclear whether cellular responses to antibiotics have a similar protective role. By measuring the genome-wide transcriptional response dynamics of Escherichia coli to four antibiotics, we found that trimethoprim induces a rapid acid stress response that protects bacteria from subsequent exposure to acid. Combining microfluidics with time-lapse imaging to monitor survival and acid stress response in single cells revealed that the noisy expression of the acid resistance operon gadBC correlates with single-cell survival. Cells with highergadBCexpression following trimethoprim maintain higher intracellular pH and survive the acid stress longer. The seemingly randomsingle-cell survival under acid stress can therefore be predicted from gadBC expression and rationalized in terms of GadB/C molecular function. Overall, we provide a roadmap for identifying the molecular mechanisms of single-cell cross-protection between antibiotics and other stressors.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mitosch, Karin UNSPECIFIED UNSPECIFIED UNSPECIFIED Rieckh, Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED Bollenbach, Tobias UNSPECIFIED orcid.org/0000-0003-4398-476X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-233667
DOI:	10.1016/j.cels.2017.03.001
Journal or Publication Title:	Cell Syst.
Volume:	4
Number:	4
Page Range:	S. 393 - 409
Date:	2017
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	2405-4720
Language:	English
Faculty:	Faculty of Law
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ESCHERICHIA-COLI O157-H7; GENE-EXPRESSION; CROSS-PROTECTION; LISTERIA-MONOCYTOGENES; ENTERIC BACTERIA; SYSTEMS BIOLOGY; RESISTANCE; PH; TRIMETHOPRIM; FLUORESCENCE Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23366