Snijder, Joost, Schuller, Jan M., Wiegard, Anika ORCID: 0000-0001-9674-0510, Lossl, Philip ORCID: 0000-0001-5062-7723, Schmelling, Nicolas ORCID: 0000-0002-6981-397X, Axmann, Ilka M., Plitzko, Juergen M., Foerster, Friedrich ORCID: 0000-0002-6044-2746 and Heck, Albert J. R. (2017). Structures of the cyanobacterial circadian oscillator frozen in a fully assembled state. Science, 355 (6330). S. 1181 - 1185. WASHINGTON: AMER ASSOC ADVANCEMENT SCIENCE. ISSN 1095-9203

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Abstract

Cyanobacteria have a robust circadian oscillator, known as the Kai system. Reconstituted from the purified protein components KaiC, KaiB, and KaiA, it can tick autonomously in the presence of adenosine 5'-triphosphate (ATP). The KaiC hexamers enter a natural 24-hour reaction cycle of autophosphorylation and assembly with KaiB and KaiA in numerous diverse forms. We describe the preparation of stoichiometrically well-defined assemblies of KaiCB and KaiCBA, as monitored by native mass spectrometry, allowing for a structural characterization by single-particle cryo-electron microscopy and mass spectrometry. Our data reveal details of the interactions between the Kai proteins and provide a structural basis to understand periodic assembly of the protein oscillator.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Snijder, JoostUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schuller, Jan M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wiegard, AnikaUNSPECIFIEDorcid.org/0000-0001-9674-0510UNSPECIFIED
Lossl, PhilipUNSPECIFIEDorcid.org/0000-0001-5062-7723UNSPECIFIED
Schmelling, NicolasUNSPECIFIEDorcid.org/0000-0002-6981-397XUNSPECIFIED
Axmann, Ilka M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Plitzko, Juergen M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Foerster, FriedrichUNSPECIFIEDorcid.org/0000-0002-6044-2746UNSPECIFIED
Heck, Albert J. R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-236484
DOI: 10.1126/science.aag3218
Journal or Publication Title: Science
Volume: 355
Number: 6330
Page Range: S. 1181 - 1185
Date: 2017
Publisher: AMER ASSOC ADVANCEMENT SCIENCE
Place of Publication: WASHINGTON
ISSN: 1095-9203
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CLOCK PROTEIN KAIB; CRYSTAL-STRUCTURE; PHOSPHORYLATION; DYNAMICS; COMPLEX; KAIABC; RHYTHM; EXPRESSIONMultiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/23648

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