Universität zu Köln

Ali, Tamer, Krueger, Marcus ORCID: 0000-0003-2008-4582, Bhuju, Sabin, Jarek, Michael, Bartkuhn, Marek ORCID: 0000-0001-6872-9082 and Renkawitz, Rainer ORCID: 0000-0002-2591-1332 (2017). Chromatin binding of Gcn5 in Drosophila is largely mediated by CP190. Nucleic Acids Res., 45 (5). S. 2384 - 2396. OXFORD: OXFORD UNIV PRESS. ISSN 1362-4962

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Abstract

Centrosomal 190 kDa protein (CP190) is a promoter binding factor, mediates long-range interactions in the context of enhancer-promoter contacts and in chromosomal domain formation. All Drosophila insulator proteins bind CP190 suggesting a crucial role in insulator function. CP190 has major effects on chromatin, such as depletion of nucleosomes, high nucleosomal turnover and prevention of heterochromatin expansion. Here, we searched for enzymes, which might be involved in CP190 mediated chromatin changes. Eighty percent of the genomic binding sites of the histone acetyltransferase Gcn5 are colocalizing with CP190 binding. Depletion of CP190 reduces Gcn5 binding to chromatin. Binding dependency was further supported by Gcn5 mediated co-precipitation of CP190. Gcn5 is known to activate transcription by histone acetylation. We used the dCas9 system to target CP190 or Gcn5 to a Polycomb repressed and H3K27me3 marked gene locus. Both, CP190 as well as Gcn5, activate this locus, thus supporting the model that CP190 recruits Gcn5 and thereby activates chromatin.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ali, Tamer UNSPECIFIED UNSPECIFIED UNSPECIFIED Krueger, Marcus UNSPECIFIED orcid.org/0000-0003-2008-4582 UNSPECIFIED Bhuju, Sabin UNSPECIFIED UNSPECIFIED UNSPECIFIED Jarek, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Bartkuhn, Marek UNSPECIFIED orcid.org/0000-0001-6872-9082 UNSPECIFIED Renkawitz, Rainer UNSPECIFIED orcid.org/0000-0002-2591-1332 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-236504
DOI:	10.1093/nar/gkw1178
Journal or Publication Title:	Nucleic Acids Res.
Volume:	45
Number:	5
Page Range:	S. 2384 - 2396
Date:	2017
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1362-4962
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GENE-EXPRESSION; PROTEIN; SAGA; INSULATORS; COMPLEXES; SITES; ACETYLATION; RNA Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23650