Universität zu Köln

Frese, Christian K., Mikhaylova, Marina ORCID: 0000-0001-7646-1346, Stucchi, Riccardo, Gautier, Violette, Liu, Qingyang, Mohammed, Shabaz ORCID: 0000-0003-2640-9560, Heck, Albert J. R., Altelaar, A. F. Maarten and Hoogenraad, Casper C. (2017). Quantitative Map of Proteome Dynamics during Neuronal Differentiation. Cell Reports, 18 (6). S. 1527 - 1543. CAMBRIDGE: CELL PRESS. ISSN 2211-1247

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Abstract

Neuronal differentiation is a multistep process that shapes and re-shapes neurons by progressing through several typical stages, including axon outgrowth, dendritogenesis, and synapse formation. To systematically profile proteome dynamics throughout neuronal differentiation, we took cultured rat hippocampal neurons at different developmental stages and monitored changes in protein abundance using a combination of stable isotope labeling and high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS). Almost one third of all 4,500 proteins quantified underwent a more than 2-fold expression change during neuronal differentiation, indicating extensive remodeling of the neuron proteome. To highlight the strength of our resource, we studied the neural-cell-adhesion molecule 1 (NCAM1) and found that it stimulates dendritic arbor development by promoting actin filament growth at the dendritic growth cone. We anticipate that our quantitative map of neuronal proteome dynamics is a rich resource for further analyses of the many identified proteins in various neurodevelopmental processes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Frese, Christian K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mikhaylova, Marina UNSPECIFIED orcid.org/0000-0001-7646-1346 UNSPECIFIED Stucchi, Riccardo UNSPECIFIED UNSPECIFIED UNSPECIFIED Gautier, Violette UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Qingyang UNSPECIFIED UNSPECIFIED UNSPECIFIED Mohammed, Shabaz UNSPECIFIED orcid.org/0000-0003-2640-9560 UNSPECIFIED Heck, Albert J. R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Altelaar, A. F. Maarten UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoogenraad, Casper C. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-239924
DOI:	10.1016/j.celrep.2017.01.025
Journal or Publication Title:	Cell Reports
Volume:	18
Number:	6
Page Range:	S. 1527 - 1543
Date:	2017
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	2211-1247
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELL-ADHESION MOLECULES; TRANSCRIPTION FACTORS; ADULT NEUROGENESIS; HIPPOCAMPUS; EXPRESSION; BRAIN; PLASTICITY; COMPLEXES; CULTURE; GROWTH Multiple languages Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23992