Universität zu Köln

Kovacs, Judit, Poor, Peter ORCID: 0000-0002-4539-6358, Kaschani, Farnusch, Chandrasekar, Balakumaran, Hong, Tram N., Misas-Villamil, Johana C., Xin, Bo T., Kaiser, Markus, Overkleeft, Herman S., Tari, Irma and van der Hoorn, Renier A. L. (2017). Proteasome Activity Profiling Uncovers Alteration of Catalytic beta 2 and beta 5 Subunits of the Stress-Induced Proteasome during Salinity Stress in Tomato Roots. Front. Plant Sci., 8. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-462X

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Abstract

The stress proteasome in the animal kingdom facilitates faster conversion of oxidized proteins during stress conditions by incorporating different catalytic beta subunits. Plants deal with similar kind of stresses and also carry multiple paralogous genes encoding for each of the three catalytic beta subunits. Here, we investigated the existence of stress proteasomes upon abiotic stress (salt stress) in tomato roots. In contrast to Arabiclopsis thaliana, tomato has a simplified proteasome gene set with single genes encoding each 13 subunit except for two genes encoding beta 2. Using proteasome activity profiling on tomato roots during salt stress, we discovered a transient modification of the catalytic subunits of the proteasome coinciding with a loss of cell viability. This stress-induced active proteasome disappears at later time points and coincides with the need to degrade oxidized proteins during salt stress. Subunit-selective proteasome probes and MS analysis of fluorescent 2D gels demonstrated that the detected stress-induced proteasome is not caused by an altered composition of subunits in active proteasomes, but involves an increased molecular weight of both labeled beta 2 and beta 5 subunits, and an additional acidic pl shift for labeled beta 5, whilst labeled beta 1 remains mostly unchanged. Treatment with phosphatase or glycosidases did not affect the migration pattern. This stress-induced proteasome may play an important role in POD during abiotic stress.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kovacs, Judit UNSPECIFIED UNSPECIFIED UNSPECIFIED Poor, Peter UNSPECIFIED orcid.org/0000-0002-4539-6358 UNSPECIFIED Kaschani, Farnusch UNSPECIFIED UNSPECIFIED UNSPECIFIED Chandrasekar, Balakumaran UNSPECIFIED UNSPECIFIED UNSPECIFIED Hong, Tram N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Misas-Villamil, Johana C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Xin, Bo T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaiser, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Overkleeft, Herman S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Tari, Irma UNSPECIFIED UNSPECIFIED UNSPECIFIED van der Hoorn, Renier A. L. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-239965
DOI:	10.3389/fpls.2017.00107
Journal or Publication Title:	Front. Plant Sci.
Volume:	8
Date:	2017
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-462X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PROGRAMMED CELL-DEATH; PLANT DEFENSE REACTIONS; E3 UBIQUITIN LIGASES; 20S PROTEASOME; 26S PROTEASOME; SALT STRESS; HYDROLASE ACTIVITIES; CARDIAC PROTEASOMES; OXIDATIVE STRESS; SALICYLIC-ACID Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23996