Universität zu Köln

Reim, Tina, Balfanz, Sabine ORCID: 0000-0002-7082-3999, Baumann, Arnd ORCID: 0000-0001-9456-7275, Blenau, Wolfgang ORCID: 0000-0002-6874-4730, Thamm, Markus and Scheiner, Ricarda ORCID: 0000-0002-7515-4253 (2017). AmTAR2: Functional characterization of a honeybee tyramine receptor stimulating adenylyl cyclase activity. Insect Biochem. Mol. Biol., 80. S. 91 - 101. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 1879-0240

Full text not available from this repository.

Abstract

The biogenic monoamines norepinephrine and epinephrine regulate important physiological functions in vertebrates. Insects such as honeybees do not synthesize these neuroactive substances. Instead, they employ octopamine and tyramine for comparable physiological functions. These biogenic amines activate specific guanine nucleotide-binding (G) protein-coupled receptors (GPCRs). Based on pharmacological data obtained on heterologously expressed receptors, alpha- and beta-adrenergic-like octopamine receptors are better activated by octopamine than by tyramine. Conversely, GPCRs forming the type 1 tyramine receptor Glade (synonymous to octopamine/tyramine receptors) are better activated by tyramine than by octopamine. More recently, receptors were characterized which are almost exclusively activated by tyramine, thus forming an independent type 2 tyramine receptor Glade. Functionally, type 1 tyramine receptors inhibit adenylyl cyclase activity, leading to a decrease in intracellular cAMP concentration ([cAMP](i)). Type 2 tyramine receptors can mediate Ca2+ signals or both Ca2+ signals and effects on [CAMP](i). We here provide evidence that the honeybee tyramine receptor 2 (AmTAR2), when heterologously expressed in flpTM cells, exclusively causes an increase in [cAMP](i). The receptor displays a pronounced preference for tyramine over octopamine. Its activity can be blocked by a series of established antagonists, of which mianserin and yohimbine are most efficient. The functional characterization of two tyramine receptors from the honeybee, AmTAR1 (previously named AmTYR1) and AmTAR2, which respond to tyramine by changing CAMP levels in opposite direction, is an important step towards understanding the actions of tyramine in honeybee behavior and physiology, particularly in comparison to the effects of octopamine. (C) 2016 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Reim, Tina UNSPECIFIED UNSPECIFIED UNSPECIFIED Balfanz, Sabine UNSPECIFIED orcid.org/0000-0002-7082-3999 UNSPECIFIED Baumann, Arnd UNSPECIFIED orcid.org/0000-0001-9456-7275 UNSPECIFIED Blenau, Wolfgang UNSPECIFIED orcid.org/0000-0002-6874-4730 UNSPECIFIED Thamm, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Scheiner, Ricarda UNSPECIFIED orcid.org/0000-0002-7515-4253 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-243485
DOI:	10.1016/j.ibmb.2016.12.004
Journal or Publication Title:	Insect Biochem. Mol. Biol.
Volume:	80
Page Range:	S. 91 - 101
Date:	2017
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication:	OXFORD
ISSN:	1879-0240
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language APIS-MELLIFERA BRAIN; PROTEIN-COUPLED-RECEPTORS; DIVISION-OF-LABOR; DROSOPHILA-MELANOGASTER; OCTOPAMINE RECEPTOR; SIGNALING PROPERTIES; BEE; EXPRESSION; BEHAVIOR; GENE Multiple languages Biochemistry & Molecular Biology; Entomology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/24348