Universität zu Köln

Wang, Lu, Cui, Yurong, Liu, Qinghua, Song, Yuanlong, Hu, Qinghua, Tang, Ming, Hescheler, Juergen and Xi, Jiaoya (2017). Puerarin Enhances Ca2+ Reuptake and Ca2+ Content of Sarcoplasmic Reticulum in Murine Embryonic Stem Cell-Derived Cardiomyocytes via Upregulation of SERCA2a. Cell. Physiol. Biochem., 44 (3). S. 1199 - 1213. BASEL: KARGER. ISSN 1421-9778

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Abstract

Background/Aims: The embryonic stem cell-derived cardiomyocytes (ES-CMs) serve as potential sources for cardiac regenerative therapy. However, the immature sarcoplasmic reticulum (SR) function of ES-CMs prevents its application. In this report, we examined the effect of puerarin, an isoflavone compound, on SR function of murine ES-CMs. Methods: Murine ES-CMs were harvested by embryoid body-based differentiation method. Confocal calcium imaging and whole-cell patch clamps were performed to assess the function of SR. The mRNA expression levels of SR-related genes were examined by quantitative PCR. The protein expression of sarcoplasmic reticulum calcium-ATPase 2a (SERCA2a) was evaluated by immunofluorescent and western blot. Results: Long-term application of puerarin promotes basic properties of spontaneous calcium transient with increased amplitude, decay velocity, and decreased duration. Puerarin fails to alter I-Ca,I-L but increases the Ca2+ content of SR. Puerarintreated ES-CMs have intact SR Ca2+ cycling with more SR Ca2+ reuptake. Long-term application of puerarin asynchronously upregulates the mRNA and protein expression of SERCA2a, as well as the transcripts of calsequestrin and triadin in developing ES-CMs. Application of puerarin during the stage of post-cardiac differentiation upregulates dose-dependently the transcripts of SERCA2a, phospholamban and tridin which can be reversed by the inhibitors of the PI3K/Akt and MAPK/ERK signaling pathways, but shows no effect on the protein expression of SERCA2a. Conclusion: This study demonstrates that long-term puerarin treatment enhances Ca2+ reuptake and Ca2+ content via upregulation of SERCA2a. (C) 2017 The Author(s) Published by S. Karger AG, Basel

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wang, Lu UNSPECIFIED UNSPECIFIED UNSPECIFIED Cui, Yurong UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Qinghua UNSPECIFIED UNSPECIFIED UNSPECIFIED Song, Yuanlong UNSPECIFIED UNSPECIFIED UNSPECIFIED Hu, Qinghua UNSPECIFIED UNSPECIFIED UNSPECIFIED Tang, Ming UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Xi, Jiaoya UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-244710
DOI:	10.1159/000485450
Journal or Publication Title:	Cell. Physiol. Biochem.
Volume:	44
Number:	3
Page Range:	S. 1199 - 1213
Date:	2017
Publisher:	KARGER
Place of Publication:	BASEL
ISSN:	1421-9778
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RAT VENTRICULAR MYOCYTES; CARDIAC CONTRACTILITY; HEART-FAILURE; MATURATION; EXPRESSION; SURVIVAL; MECHANISM; EXERTS Multiple languages Cell Biology; Physiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/24471