Universität zu Köln

Albanna, Walid ORCID: 0000-0001-9986-8739, Lueke, Jan Niklas, Sjapic, Volha, Kotliar, Konstantin ORCID: 0000-0003-2087-957X, Hescheler, Juergen, Clusmann, Hans, Sjapic, Sergej, Alpdogan, Serdar ORCID: 0000-0002-5188-9925, Schneider, Toni, Schubert, Gerrit Alexander ORCID: 0000-0001-9135-6042 and Neumaier, Felix ORCID: 0000-0002-6376-6391 (2017). Electroretinographic Assessment of Inner Retinal Signaling in the Isolated and Superfused Murine Retina. Curr. Eye Res., 42 (11). S. 1518 - 1527. PHILADELPHIA: TAYLOR & FRANCIS INC. ISSN 1460-2202

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Abstract

Purpose: Longer-lasting electroretinographic recordings of the isolated murine retina were initially achieved by modification of a phosphate-buffered nutrient solution originally developed for the bovine retina. During experiments with a more sensitive mouse retina, apparent model-specific limitations were addressed and improvements were analyzed for their contribution to an optimized full electroretinogram (ERG).Material and methods: Retinas were isolated from dark-adapted mice, transferred to a recording chamber and superfused with different solutions. Scotopic and photopic ERGs were recorded with white flashes every 3 minutes. The phosphate buffer (Sickel-medium) originally used was replaced by a carbonate-based system (Ames-medium), the pH of which was adjusted to 7.7-7.8. Moreover, addition of 0.1 mM BaCl2 was investigated to reduce b-wave contamination by the slow PIII component typically present in the murine ERG.Results: B-wave amplitudes were increased by the pH-shift (pH 7.4 to pH 7.7) from 22.9 1.9 mu V to 37.5 +/- 2.5 mu V. Improved b-wave responses were also achieved by adding small amounts of Ba2+ (100 mu M), which selectively suppressed slow PIII components, thereby unmasking more of the true b-wave amplitude (100.0% with vs. 22.2 +/- 10.7% without Ba2+). Ames medium lacking amino acids and vitamins was unable to maintain retinal signaling, as evident in a reversible decrease of the b-wave to 31.8 +/- 3.9% of its amplitude in complete Ames medium.Conclusions: Our findings provide optimized conditions for ex vivo ERGs from the murine retina and suggest that careful application of Ba2+ supports reliable isolation of b-wave responses in mice. Under our recording conditions, murine retinas show reproducible ERGs for up to six hours.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Albanna, Walid UNSPECIFIED orcid.org/0000-0001-9986-8739 UNSPECIFIED Lueke, Jan Niklas UNSPECIFIED UNSPECIFIED UNSPECIFIED Sjapic, Volha UNSPECIFIED UNSPECIFIED UNSPECIFIED Kotliar, Konstantin UNSPECIFIED orcid.org/0000-0003-2087-957X UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Clusmann, Hans UNSPECIFIED UNSPECIFIED UNSPECIFIED Sjapic, Sergej UNSPECIFIED UNSPECIFIED UNSPECIFIED Alpdogan, Serdar UNSPECIFIED orcid.org/0000-0002-5188-9925 UNSPECIFIED Schneider, Toni UNSPECIFIED UNSPECIFIED UNSPECIFIED Schubert, Gerrit Alexander UNSPECIFIED orcid.org/0000-0001-9135-6042 UNSPECIFIED Neumaier, Felix UNSPECIFIED orcid.org/0000-0002-6376-6391 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-244915
DOI:	10.1080/02713683.2017.1339807
Journal or Publication Title:	Curr. Eye Res.
Volume:	42
Number:	11
Page Range:	S. 1518 - 1527
Date:	2017
Publisher:	TAYLOR & FRANCIS INC
Place of Publication:	PHILADELPHIA
ISSN:	1460-2202
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Medicine > Physiologie und Pathophysiologie > Institut für Neurophysiologie
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SOURCE-DENSITY ANALYSIS; GATED CA2+ CHANNELS; ISOLATED RAT RETINA; B-WAVE AMPLITUDE; VERTEBRATE RETINA; FIELD POTENTIALS; BOVINE RETINA; ERG; STIMULATION; THRESHOLD Multiple languages Ophthalmology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/24491