Universität zu Köln

Lauterbach, Maximilian ORCID: 0000-0002-9450-5485, Billakurthi, Kumari, Kadereit, Gudrun, Ludwig, Martha ORCID: 0000-0002-0324-7602, Westhoff, Peter and Gowik, Udo ORCID: 0000-0002-8986-7318 (2017). C-3 cotyledons are followed by C-4 leaves: intra-individual transcriptome analysis of Salsola soda Chenopodiaceae). J. Exp. Bot., 68 (2). S. 161 - 177. OXFORD: OXFORD UNIV PRESS. ISSN 1460-2431

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Abstract

Some species of Salsoleae (Chenopodiaceae) convert from C-3 photosynthesis during the seedling stage to the C-4 pathway in adult leaves. This unique developmental transition of photosynthetic pathways offers the exceptional opportunity to follow the development of the derived C-4 syndrome from the C-3 condition within individual plants, avoiding phylogenetic noise. Here we investigate Salsola soda, a little-studied species from tribe Salsoleae, using an ontogenetic approach. Anatomical sections, carbon isotope (delta C-13) values, transcriptome analysis by means of mRNA sequencing, and protein levels of the key C-4 enzyme phosphoenolpyruvate carboxylase (PEPC) were examined from seed to adult plant stages. Despite a previous report, our results based on delta C-13 values, anatomy and transcriptomics clearly indicate a C-3 phase during the cotyledon stage. During this stage, the entire transcriptional repertoire of the C-4 NADP-malic enzyme type is detected at low levels compared to a significant increase in true leaves. In contrast, abundance of transcripts encoding most of the major photorespiratory enzymes is not significantly decreased in leaves compared to cotyledons. PEPC polypeptide was detected only in leaves, correlating with increased PEPC transcript abundance from the cotyledon to leaf stage.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lauterbach, Maximilian UNSPECIFIED orcid.org/0000-0002-9450-5485 UNSPECIFIED Billakurthi, Kumari UNSPECIFIED UNSPECIFIED UNSPECIFIED Kadereit, Gudrun UNSPECIFIED UNSPECIFIED UNSPECIFIED Ludwig, Martha UNSPECIFIED orcid.org/0000-0002-0324-7602 UNSPECIFIED Westhoff, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Gowik, Udo UNSPECIFIED orcid.org/0000-0002-8986-7318 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-246816
DOI:	10.1093/jxb/erw343
Journal or Publication Title:	J. Exp. Bot.
Volume:	68
Number:	2
Page Range:	S. 161 - 177
Date:	2017
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1460-2431
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CARBON-ISOTOPE DISCRIMINATION; BUNDLE-SHEATH CHLOROPLASTS; GENE-EXPRESSION; PHOSPHOENOLPYRUVATE CARBOXYKINASE; DIFFERENTIAL EXPRESSION; MOLECULAR PHYLOGENY; LEAF SENESCENCE; MESSENGER-RNA; PHOTOSYNTHESIS; EVOLUTION Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/24681