Universität zu Köln

Milicevic, Novica M., Nohroudi, Klaus, Schmidt, Friederike, Schmidt, Hendrik, Ringer, Cornelia, Sorensen, Grith Lykke ORCID: 0000-0002-5273-1097, Milicevic, Zivana and Westermann, Juergen (2016). Growth of Murine Splenic Tissue Is Suppressed by Lymphotoxin beta-Receptor Signaling (LT beta R) Originating from Splenic and Non-Splenic Tissues. PLoS One, 11 (12). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

Development and maintenance of secondary lymphoid organs such as lymph nodes and spleen essentially depend on lymphotoxin beta-receptor (LT beta R) signaling. It is unclear, however, by which molecular mechanism their size is limited. Here, we investigate whether the LT beta R pathway is also growth suppressing. By using splenic tissue transplantation it is possible to analyze a potential contribution of LT beta R signaling inside and outside of the implanted tissue. We show that LT beta R signaling within the endogenous spleen and within non-splenic tissues both significantly suppressed the regeneration of implanted splenic tissue. The suppressive activity positively correlated with the total number of LT beta R expressing cells in the animal (regenerate weights of 115 +/- 8 mg in LT beta R deficient recipients and of 12 +/- 9 mg in wild-type recipients), affected also developed splenic tissue, and was induced but not executed via LT beta R signaling. Two-dimensional differential gel electrophoresis and subsequent mass spectrometry of stromal splenic tissue was applied to screen for potential factors mediating the LT beta R dependent suppressive activity. Thus, LT beta R dependent growth suppression is involved in regulating the size of secondary lymphoid organs, and might be therapeutically used to eradicate tertiary lymphoid tissues during autoimmune diseases.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Milicevic, Novica M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Nohroudi, Klaus UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Friederike UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Hendrik UNSPECIFIED UNSPECIFIED UNSPECIFIED Ringer, Cornelia UNSPECIFIED UNSPECIFIED UNSPECIFIED Sorensen, Grith Lykke UNSPECIFIED orcid.org/0000-0002-5273-1097 UNSPECIFIED Milicevic, Zivana UNSPECIFIED UNSPECIFIED UNSPECIFIED Westermann, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-252656
DOI:	10.1371/journal.pone.0166901
Journal or Publication Title:	PLoS One
Volume:	11
Number:	12
Date:	2016
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MICROFIBRIL-ASSOCIATED GLYCOPROTEIN; TUMOR-NECROSIS-FACTOR; T-CELL HOMEOSTASIS; LYMPH-NODE; SECRETORY LYSOSOMES; MESSENGER-RNA; PROTEIN 4; EXPRESSION; SECONDARY; MFAP4 Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/25265