Universität zu Köln

van Setten, Gysbert B., Trost, Andrea, Schroedl, Falk, Kaser-Eichberger, Alexandra, Bogner, Barbara, van Setten, Mercedes, Heindl, Ludwig M., Grabner, Guenther and Reitsamer, Herbert A. (2016). Immunohistochemical Detection of CTGF in the Human Eye. Curr. Eye Res., 41 (12). S. 1571 - 1580. PHILADELPHIA: TAYLOR & FRANCIS INC. ISSN 1460-2202

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Abstract

Purpose/Aim of the study: Connective tissue growth factor (CTGF) is a key player in the control of extracellular matrix remodeling, fibrosis, and angiogenesis. It is also involved in the modification of the trabecular meshwork, thus potentially modulating outflow facility and intraocular pressure (IOP). As a consequence, CTGF might be relevant for the development of elevated IOP, a major risk factor in glaucoma-pathogenesis. While comprehensive information on the origins of CTGF in the human eye is not available, the goal of this study is to identify ocular sources of CTGF using morphological methods.Materials and Methods: Human donor eyes were prepared for immunohistochemical analysis of CTGF, -smooth muscle-actin (ASMA), and CD31. Confocal laser scanning microscopy was used for documentation.Results: In the cornea, CTGF-immunoreactivity (CTGF-IR) was detected in the epithelium, mainly in basal layers, stromal keratinocytes, and endothelial cells. Adjacent conjunctiva showed also CTGF-IR in epithelial cells. In the iris, both, the sphincter and dilator muscles displayed CGTF-IR, as did iris and ciliary body vessels, deriving at this location from the vascular endothelium, as detected with CD31, but not from vascular smooth muscle cells, as detected with ASMA. In the ciliary body, CTGF-IR was detected in smooth-muscle cells of the ciliary muscle and further in the non-pigmented epithelium. In the retina, CTGF-IR was detected in the NFL and weakly in the IPL/OPL. In the choroid, the choriocapillaris and blood vessels displayed CTGF-IR. Further, few cells in the optic nerve head and the lamina cribrosa were CTGF-positive.Conclusion: CTGF was detected in various structures of the human eye. Since CTGF has been also described in aqueous humor, the identified structures might be the sources of CTGF in the aqueous humor. By means of aqueous flow, CTGF is transported into the trabecular meshwork, where it could change outflow facility and therefore affecting IOP homeostasis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code van Setten, Gysbert B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Trost, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Schroedl, Falk UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaser-Eichberger, Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Bogner, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED van Setten, Mercedes UNSPECIFIED UNSPECIFIED UNSPECIFIED Heindl, Ludwig M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Grabner, Guenther UNSPECIFIED UNSPECIFIED UNSPECIFIED Reitsamer, Herbert A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-253937
DOI:	10.3109/02713683.2016.1143014
Journal or Publication Title:	Curr. Eye Res.
Volume:	41
Number:	12
Page Range:	S. 1571 - 1580
Date:	2016
Publisher:	TAYLOR & FRANCIS INC
Place of Publication:	PHILADELPHIA
ISSN:	1460-2202
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TISSUE-GROWTH-FACTOR; TRABECULAR MESHWORK; MATRICELLULAR PROTEINS; EXTRACELLULAR-MATRIX; LAMINA-CRIBROSA; TGF-BETA; FACTOR EXPRESSION; EPITHELIAL-CELLS; UP-REGULATION; CCN FAMILY Multiple languages Ophthalmology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/25393