Universität zu Köln

Dursun, Ezgi, Endele, Max, Musumeci, Andrea ORCID: 0000-0002-2497-0699, Failmezger, Henrik, Wang, Shu-Hung, Tresch, Achim, Schroeder, Timm and Krug, Anne B. (2016). Continuous single cell imaging reveals sequential steps of plasmacytoid dendritic cell development from common dendritic cell progenitors. Sci Rep, 6. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

Functionally distinct plasmacytoid and conventional dendritic cells (pDC and cDC) shape innate and adaptive immunity. They are derived from common dendritic cell progenitors (CDPs) in the murine bone marrow, which give rise to CD11c(+) MHCII- precursors with early commitment to DC subpopulations. In this study, we dissect pDC development from CDP into an ordered sequence of differentiation events by monitoring the expression of CD11c, MHC class II, Siglec H and CCR9 in CDP cultures by continuous single cell imaging and tracking. Analysis of CDP genealogies revealed a stepwise differentiation of CDPs into pDCs in a part of the CDP colonies. This developmental pathway involved an early CD11c(+) SiglecH(-) pre-DC stage and a Siglec H+ CCR9(low) precursor stage, which was followed rapidly by upregulation of CCR9 indicating final pDC differentiation. In the majority of the remaining CDP pedigrees however the Siglec H+ CCR9(low) precursor state was maintained for several generations. Thus, although a fraction of CDPs transits through precursor stages rapidly to give rise to a first wave of pDCs, the majority of CDP progeny differentiate more slowly and give rise to longer lived precursor cells which are poised to differentiate on demand.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Dursun, Ezgi UNSPECIFIED UNSPECIFIED UNSPECIFIED Endele, Max UNSPECIFIED UNSPECIFIED UNSPECIFIED Musumeci, Andrea UNSPECIFIED orcid.org/0000-0002-2497-0699 UNSPECIFIED Failmezger, Henrik UNSPECIFIED UNSPECIFIED UNSPECIFIED Wang, Shu-Hung UNSPECIFIED UNSPECIFIED UNSPECIFIED Tresch, Achim UNSPECIFIED UNSPECIFIED UNSPECIFIED Schroeder, Timm UNSPECIFIED UNSPECIFIED UNSPECIFIED Krug, Anne B. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-255362
DOI:	10.1038/srep37462
Journal or Publication Title:	Sci Rep
Volume:	6
Date:	2016
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TRANSCRIPTION FACTOR E2-2; BONE-MARROW; EXPRESSION; SUBSETS; BLOOD; PDCS; IDENTIFICATION; HETEROGENEITY; PHENOTYPE; DEPLETION Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/25536