Pauli, Thomas, Vedder, Lucia ORCID: 0000-0002-8924-9800, Dowling, Daniel, Petersen, Malte ORCID: 0000-0001-7601-9873, Meusemann, Karen, Donath, Alexander ORCID: 0000-0001-5618-0547, Peters, Ralph S., Podsiadlowski, Lars ORCID: 0000-0001-7786-8930, Mayer, Christoph ORCID: 0000-0001-5104-6621, Liu, Shanlin, Zhou, Xin, Heger, Peter ORCID: 0000-0003-2583-2981, Wiehe, Thomas ORCID: 0000-0002-8932-2772, Hering, Lars ORCID: 0000-0003-4469-991X, Mayer, Georg ORCID: 0000-0003-0737-2440, Misof, Bernhard and Niehuis, Oliver (2016). Transcriptomic data from panarthropods shed new light on the evolution of insulator binding proteins in insects. BMC Genomics, 17. LONDON: BMC. ISSN 1471-2164

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Abstract

Background: Body plan development in multi-cellular organisms is largely determined by homeotic genes. Expression of homeotic genes, in turn, is partially regulated by insulator binding proteins (IBPs). While only a few enhancer blocking IBPs have been identified in vertebrates, the common fruit fly Drosophila melanogaster harbors at least twelve different enhancer blocking IBPs. We screened recently compiled insect transcriptomes from the 1KITE project and genomic and transcriptomic data from public databases, aiming to trace the origin of IBPs in insects and other arthropods. Results: Our study shows that the last common ancestor of insects (Hexapoda) already possessed a substantial number of IBPs. Specifically, of the known twelve insect IBPs, at least three (i.e., CP190, Su(Hw), and CTCF) already existed prior to the evolution of insects. Furthermore we found GAF orthologs in early branching insect orders, including Zygentoma (silverfish and firebrats) and Diplura (two-pronged bristletails). Mod(mdg4) is most likely a derived feature of Neoptera, while Pita is likely an evolutionary novelty of holometabolous insects. Zw5 appears to be restricted to schizophoran flies, whereas BEAF-32, ZIPIC and the Elba complex, are probably unique to the genus Drosophila. Selection models indicate that insect IBPs evolved under neutral or purifying selection. Conclusions: Our results suggest that a substantial number of IBPs either pre-date the evolution of insects or evolved early during insect evolution. This suggests an evolutionary history of insulator binding proteins in insects different to that previously thought. Moreover, our study demonstrates the versatility of the 1KITE transcriptomic data for comparative analyses in insects and other arthropods.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Pauli, ThomasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vedder, LuciaUNSPECIFIEDorcid.org/0000-0002-8924-9800UNSPECIFIED
Dowling, DanielUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Petersen, MalteUNSPECIFIEDorcid.org/0000-0001-7601-9873UNSPECIFIED
Meusemann, KarenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Donath, AlexanderUNSPECIFIEDorcid.org/0000-0001-5618-0547UNSPECIFIED
Peters, Ralph S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Podsiadlowski, LarsUNSPECIFIEDorcid.org/0000-0001-7786-8930UNSPECIFIED
Mayer, ChristophUNSPECIFIEDorcid.org/0000-0001-5104-6621UNSPECIFIED
Liu, ShanlinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zhou, XinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Heger, PeterUNSPECIFIEDorcid.org/0000-0003-2583-2981UNSPECIFIED
Wiehe, ThomasUNSPECIFIEDorcid.org/0000-0002-8932-2772UNSPECIFIED
Hering, LarsUNSPECIFIEDorcid.org/0000-0003-4469-991XUNSPECIFIED
Mayer, GeorgUNSPECIFIEDorcid.org/0000-0003-0737-2440UNSPECIFIED
Misof, BernhardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Niehuis, OliverUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-255953
DOI: 10.1186/s12864-016-3205-1
Journal or Publication Title: BMC Genomics
Volume: 17
Date: 2016
Publisher: BMC
Place of Publication: LONDON
ISSN: 1471-2164
Language: English
Faculty: Faculty of Mathematics and Natural Sciences
Divisions: Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ENHANCER BLOCKING ACTIVITY; DROSOPHILA-MELANOGASTER; CHROMATIN INSULATOR; GENOME SEQUENCE; DOMAIN BOUNDARY; ZINC FINGERS; GAGA FACTOR; GENES; PROMOTER; ELEMENTMultiple languages
Biotechnology & Applied Microbiology; Genetics & HeredityMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/25595

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