Universität zu Köln

Burg, M. C., Lunkenheimer, P., Niederer, P., Brune, C., Redmann, K., Smerup, M., Spiegel, U., Becker, F., Maintz, D., Heindel, W. and Anderson, R. H. (2016). Pneumatic Distension of Ventricular Mural Architecture Validated Histologically. Rofo-Fortschr. Gebiet Rontgenstrahlen Bildgeb. Verfahr., 188 (11). S. 1045 - 1054. STUTTGART: GEORG THIEME VERLAG KG. ISSN 1438-9010

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Abstract

Purpose: There are ongoing arguments as to how cardiomyocytes are aggregated together within the ventricular walls. We used pneumatic distension through the coronary arteries to exaggerate the gaps between the aggregated cardiomyocytes, analyzing the pattern revealed using computed tomography, and validating our findings by histology. Methods: We distended 10 porcine hearts, arresting 4 in diastole by infusion of cardioplegic solutions, and 4 in systole by injection of barium chloride. Mural architecture was revealed by computed tomography, measuring also the angulations of the long chains of cardiomyocytes. We prepared the remaining 2 hearts for histology by perfusion with formaldehyde. Results: Increasing pressures of pneumatic distension elongated the ventricular walls, but produced insignificant changes in mural thickness. The distension exaggerated the spaces between the aggregated cardiomyocytes, compartmenting the walls into epicardial, central, and endocardial regions, with a feathered arrangement of transitions between them. Marked variation was noted in the thicknesses of the parts in the different ventricular segments, with no visible anatomical boundaries between them. Measurements of angulations revealed intruding and extruding populations of cardiomyocytes that deviated from a surface-parallel alignment. Scrolling through the stacks of tomographic images revealed marked spiraling of the aggregated cardiomyocytes when traced from base to apex. Conclusion: Our findings call into question the current assumption that cardiomyocytes are uniformly aggregated together in a tangential fashion. There is marked heterogeneity in the architecture of the different ventricular segments, with the aggregated units never extending in a fully transmural fashion.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Burg, M. C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lunkenheimer, P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Niederer, P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Brune, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Redmann, K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Smerup, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Spiegel, U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Maintz, D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Heindel, W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Anderson, R. H. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-257183
DOI:	10.1055/s-0042-115569
Journal or Publication Title:	Rofo-Fortschr. Gebiet Rontgenstrahlen Bildgeb. Verfahr.
Volume:	188
Number:	11
Page Range:	S. 1045 - 1054
Date:	2016
Publisher:	GEORG THIEME VERLAG KG
Place of Publication:	STUTTGART
ISSN:	1438-9010
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language COMPUTED-TOMOGRAPHY; CARDIAC FIBER; DIFFUSION; MYOCARDIUM; HEART; WALL; MRI; MYOCYTES Multiple languages Radiology, Nuclear Medicine & Medical Imaging Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/25718