Fatima, Azra, Xu, Guoxing, Nguemo, Filomain, Kuzmenkin, Alexey, Burkert, Karsten, Hescheler, Juergen and Saric, Tomo ORCID: 0000-0001-8344-1095
(2016).
Murine transgenic iPS cell line for monitoring and selection of cardiomyocytes.
Stem Cell Res., 17 (2).
S. 266 - 273.
AMSTERDAM:
ELSEVIER SCIENCE BV.
ISSN 1876-7753
Abstract
We report here a transgenic murine induced pluripotent stem cell (iPSC) line expressing puromycin N-acetyl-transferase (PAC) and enhanced green fluorescent protein (EGFP) under the control of a-myosin heavy chain promoter. This transgenic cell line reproducibly differentiates into EGFP-expressing cardiomyocytes (CMs) which can be generated at high purity with puromycin treatment and exhibit molecular and functional properties of immature heart muscle cells. This genetically modified iPSC line can be used for assessment of the utility of CMs for myocardial repair, pharmacological and toxicological applications and development of improved cardiac differentiation protocols. (C) 2016 The Authors. Published by Elsevier B.V.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||||
Creators: |
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URN: | urn:nbn:de:hbz:38-263125 | ||||||||||||||||||||||||||||||||
DOI: | 10.1016/j.scr.2016.07.007 | ||||||||||||||||||||||||||||||||
Journal or Publication Title: | Stem Cell Res. | ||||||||||||||||||||||||||||||||
Volume: | 17 | ||||||||||||||||||||||||||||||||
Number: | 2 | ||||||||||||||||||||||||||||||||
Page Range: | S. 266 - 273 | ||||||||||||||||||||||||||||||||
Date: | 2016 | ||||||||||||||||||||||||||||||||
Publisher: | ELSEVIER SCIENCE BV | ||||||||||||||||||||||||||||||||
Place of Publication: | AMSTERDAM | ||||||||||||||||||||||||||||||||
ISSN: | 1876-7753 | ||||||||||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||||||||||
Uncontrolled Keywords: |
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Refereed: | Yes | ||||||||||||||||||||||||||||||||
URI: | http://kups.ub.uni-koeln.de/id/eprint/26312 |
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