Universität zu Köln

Hepp, Christof, Gangel, Heike, Henseler, Katja, Guenther, Niklas and Maier, Berenike ORCID: 0000-0001-6971-9927 (2016). Single-Stranded DNA Uptake during Gonococcal Transformation. J. Bacteriol., 198 (18). S. 2515 - 2524. WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1098-5530

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Abstract

Neisseria gonorrhoeae is naturally competent for transformation. The first step of the transformation process is the uptake of DNA from the environment into the cell. This transport step is driven by a powerful molecular machine. Here, we addressed the question whether this machine imports single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) at similar rates. The fluorescence signal associated with the uptake of short DNA fragments labeled with a single fluorescent marker molecule was quantified. We found that ssDNA with a double-stranded DNA uptake sequence (DUS) was taken up with a similar efficiency as dsDNA. Imported ssDNA was degraded rapidly, and the thermonuclease Nuc was required for degradation. In a nuc deletion background, dsDNA and ssDNA with a double-stranded DUS were imported and used as the substrates for transformation, whereas the import and transformation efficiencies of ssDNA with single-stranded DUS were below the detection limits. We conclude that the DNA uptake machine requires a double-stranded DUS for efficient DNA recognition and transports ssDNA and dsDNA with comparable efficiencies.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hepp, Christof UNSPECIFIED UNSPECIFIED UNSPECIFIED Gangel, Heike UNSPECIFIED UNSPECIFIED UNSPECIFIED Henseler, Katja UNSPECIFIED UNSPECIFIED UNSPECIFIED Guenther, Niklas UNSPECIFIED UNSPECIFIED UNSPECIFIED Maier, Berenike UNSPECIFIED orcid.org/0000-0001-6971-9927 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-264144
DOI:	10.1128/JB.00464-16
Journal or Publication Title:	J. Bacteriol.
Volume:	198
Number:	18
Page Range:	S. 2515 - 2524
Date:	2016
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	1098-5530
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Physics > Institut für Biologische Physik
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IV PILUS EXPRESSION; NEISSERIA-GONORRHOEAE; NATURAL TRANSFORMATION; GENETIC-TRANSFORMATION; COMPETENCE; BINDING; PILIN; TRANSLOCATION; BIOGENESIS; COMPONENTS Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/26414