Universität zu Köln

Couto, Daniel ORCID: 0000-0003-0223-1497, Niebergall, Roda, Liang, Xiangxiu, Bucherl, Christoph A., Sklenar, Jan, Macho, Alberto P., Ntoukakis, Vardis ORCID: 0000-0003-0069-6004, Derbyshire, Paul, Altenbach, Denise, Maclean, Dan, Robatzek, Silke, Uhrig, Joachim, Menke, Frank ORCID: 0000-0003-2490-4824, Zhou, Jian-Min ORCID: 0000-0002-9943-2975 and Zipfel, Cyril (2016). The Arabidopsis Protein Phosphatase PP2C38 Negatively Regulates the Central Immune Kinase BIK1. PLoS Pathog., 12 (8). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1553-7374

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Abstract

Plants recognize pathogen-associated molecular patterns (PAMPs) via cell surface-localized pattern recognition receptors (PRRs), leading to PRR-triggered immunity (PTI). The Arabidopsis cytoplasmic kinase BIK1 is a downstream substrate of several PRR complexes. How plant PTI is negatively regulated is not fully understood. Here, we identify the protein phosphatase PP2C38 as a negative regulator of BIK1 activity and BIK1-mediated immunity. PP2C38 dynamically associates with BIK1, as well as with the PRRs FLS2 and EFR, but not with the co-receptor BAK1. PP2C38 regulates PAMP-induced BIK1 phosphorylation and impairs the phosphorylation of the NADPH oxidase RBOHD by BIK1, leading to reduced oxidative burst and stomatal immunity. Upon PAMP perception, PP2C38 is phosphorylated on serine 77 and dissociates from the FLS2/EFR-BIK1 complexes, enabling full BIK1 activation. Together with our recent work on the control of BIK1 turnover, this study reveals another important regulatory mechanism of this central immune component.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Couto, Daniel UNSPECIFIED orcid.org/0000-0003-0223-1497 UNSPECIFIED Niebergall, Roda UNSPECIFIED UNSPECIFIED UNSPECIFIED Liang, Xiangxiu UNSPECIFIED UNSPECIFIED UNSPECIFIED Bucherl, Christoph A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sklenar, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Macho, Alberto P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ntoukakis, Vardis UNSPECIFIED orcid.org/0000-0003-0069-6004 UNSPECIFIED Derbyshire, Paul UNSPECIFIED UNSPECIFIED UNSPECIFIED Altenbach, Denise UNSPECIFIED UNSPECIFIED UNSPECIFIED Maclean, Dan UNSPECIFIED UNSPECIFIED UNSPECIFIED Robatzek, Silke UNSPECIFIED UNSPECIFIED UNSPECIFIED Uhrig, Joachim UNSPECIFIED UNSPECIFIED UNSPECIFIED Menke, Frank UNSPECIFIED orcid.org/0000-0003-2490-4824 UNSPECIFIED Zhou, Jian-Min UNSPECIFIED orcid.org/0000-0002-9943-2975 UNSPECIFIED Zipfel, Cyril UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-267401
DOI:	10.1371/journal.ppat.1005811
Journal or Publication Title:	PLoS Pathog.
Volume:	12
Number:	8
Date:	2016
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1553-7374
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NADPH OXIDASE RBOHD; PLANT INNATE IMMUNITY; CYTOPLASMIC KINASE; PSEUDOMONAS-SYRINGAE; RECEPTOR COMPLEXES; TRIGGERED IMMUNITY; ACTIVATION; BAK1; PERCEPTION; GROWTH Multiple languages Microbiology; Parasitology; Virology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/26740