Universität zu Köln

Damm, Anna, Giebeler, Nives, Zamek, Jan, Zigrino, Paola ORCID: 0000-0002-7470-0064 and Kufer, Thomas A. (2016). Epidermal NLRP10 contributes to contact hypersensitivity responses in mice. Eur. J. Immunol., 46 (8). S. 1959 - 1970. HOBOKEN: WILEY-BLACKWELL. ISSN 1521-4141

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Abstract

The nucleotide binding and oligomerization domain-like receptor (NLR) protein NLRP10 is highly expressed in the epidermis and contributes to cell-autonomous responses against invasive bacteria. To investigate the role of NLRP10 in inflammatory responses of the skin we analyzed the effect of full-body and keratinocyte-specific depletion of NLRP10 in croton oil-induced irritant contact dermatitis (ICD) and 1-fluoro-2,4-dinitrobenzene (DNFB)-induced contact hypersensitivity (CHS) in mice. Nlrp10(-/-) mice were phenotypically normal and skin repair after woundingwas not affected by lack of NLRP10. Similarly, we did not detect a contribution of NLRP10 to the ICD response induced by croton oil. In contrast, Nlrp10(-/-) mice showed significantly reduced inflammation in the DNFB-induced CHS response as compared to control animals. Microscopic analysis revealed significantly reduced numbers of CD4(+) and CD8(+) T cells in the infiltrates of animals lacking NLRP10 expression after CHS challenge. Epidermis-specific deletion of Nlrp10 by keratin-14 promotor driven Cre-recombinase was sufficient to account for this phenotype, although lymphocyte recruitment seemed to be unaltered in animals lacking NLRP10 expression in keratinocytes. Taken together, we provide evidence that NLRP10 contributes to T-cell-mediated inflammatory responses in the skin and highlight a physiological role of NLRP10 in epidermal keratinocytes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Damm, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Giebeler, Nives UNSPECIFIED UNSPECIFIED UNSPECIFIED Zamek, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Zigrino, Paola UNSPECIFIED orcid.org/0000-0002-7470-0064 UNSPECIFIED Kufer, Thomas A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-268819
DOI:	10.1002/eji.201646401
Journal or Publication Title:	Eur. J. Immunol.
Volume:	46
Number:	8
Page Range:	S. 1959 - 1970
Date:	2016
Publisher:	WILEY-BLACKWELL
Place of Publication:	HOBOKEN
ISSN:	1521-4141
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NOD-LIKE RECEPTORS; ADAPTIVE IMMUNITY; ATOPIC-DERMATITIS; IMAGE-ANALYSIS; T-CELLS; INFLAMMATION; MECHANISMS; CASPASE-1; DISEASE; PROTEIN Multiple languages Immunology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/26881