Universität zu Köln

Saini, Vedangana ORCID: 0000-0002-9944-0262, Loers, Gabriele, Kaur, Gurcharan, Schachner, Melitta and Jakovcevski, Igor ORCID: 0000-0001-7673-9600 (2016). Impact of neural cell adhesion molecule deletion on regeneration after mouse spinal cord injury. Eur. J. Neurosci., 44 (1). S. 1734 - 1747. HOBOKEN: WILEY. ISSN 1460-9568

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Abstract

The neural cell adhesion molecule (NCAM) plays important functional roles in development of the nervous system. We investigated the influence of a constitutive ablation of NCAM on the outcome of spinal cord injury. Transgenic mice lacking NCAM (NCAM-/-) were subjected to severe compression injury of the lower thoracic spinal cord using wild-type (NCAM+/+) littermates as controls. According to the single-frame motion analysis, the NCAM-/- mice showed reduced locomotor recovery in comparison to control mice at 3 and 6weeks after injury, indicating an overall positive impact of NCAM on recovery after injury. Also the Basso Mouse Scale score was lower in NCAM-/- mice at 3weeks after injury, whereas at 6weeks after injury the difference between genotypes was not statistically significant. Worse locomotor function was associated with decreased monoaminergic and cholinergic innervation of the spinal cord caudal to the injury site and decreased axonal regrowth/sprouting at the site of injury. Astrocytic scar formation at the injury site, as assessed by immunohistology for glial fibrillary acidic protein at and around the lesion site was increased in NCAM-/- compared with NCAM+/+ mice. Migration of cultured monolayer astrocytes from NCAM-/- mice was reduced as assayed by scratch wounding. Numbers of Iba-1 immunopositive microglia were not different between genotypes. We conclude that constitutive NCAM deletion in young adult mice reduces recovery after spinal cord injury, validating the hypothesized beneficial role of this molecule in recovery after injury.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Saini, Vedangana UNSPECIFIED orcid.org/0000-0002-9944-0262 UNSPECIFIED Loers, Gabriele UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaur, Gurcharan UNSPECIFIED UNSPECIFIED UNSPECIFIED Schachner, Melitta UNSPECIFIED UNSPECIFIED UNSPECIFIED Jakovcevski, Igor UNSPECIFIED orcid.org/0000-0001-7673-9600 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-270799
DOI:	10.1111/ejn.13271
Journal or Publication Title:	Eur. J. Neurosci.
Volume:	44
Number:	1
Page Range:	S. 1734 - 1747
Date:	2016
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1460-9568
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POLYSIALIC ACID; NEURITE OUTGROWTH; AXON REGENERATION; GLIAL SCAR; RECOGNITION MOLECULES; FUNCTIONAL RECOVERY; SCHWANN-CELLS; SYNAPTIC PLASTICITY; COMPRESSION INJURY; RAT ASTROCYTES Multiple languages Neurosciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27079