Universität zu Köln

Yueksel, Melih, Power, Jeffrey J., Ribbe, Jan, Volkmann, Thorsten and Maier, Berenike ORCID: 0000-0001-6971-9927 (2016). Fitness Trade-Offs in Competence Differentiation of Bacillus subtilis. Front. Microbiol., 7. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-302X

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Abstract

In the stationary phase, Bacillus subtilis differentiates stochastically and transiently into the state of competence for transformation (K-state). The latter is associated with growth arrest, and it is unclear how the ability to develop competence is stably maintained, despite its cost. To quantify the effect differentiation has on the competitive fitness of B. subtilis, we characterized the competition dynamics between strains with different probabilities of entering the K-state. The relative fitness decreased with increasing differentiation probability both during the stationary phase and during outgrowth. When exposed to antibiotics inhibiting cell wall synthesis, transcription, and translation, cells that differentiated into the K-state showed a selective advantage compared to differentiation-deficient bacteria; this benefit did not require transformation. Although beneficial, the K-state was not induced by sub-MIC concentrations of antibiotics. Increasing the differentiation probability beyond the wt level did not significantly affect the competition dynamics with transient antibiotic exposure. We conclude that the competition dynamics are very sensitive to the fraction of competent cells under benign conditions but less sensitive during antibiotic exposure, supporting the picture of stochastic differentiation as a fitness trade-off.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Yueksel, Melih UNSPECIFIED UNSPECIFIED UNSPECIFIED Power, Jeffrey J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ribbe, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Volkmann, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Maier, Berenike UNSPECIFIED orcid.org/0000-0001-6971-9927 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-272402
DOI:	10.3389/fmicb.2016.00888
Journal or Publication Title:	Front. Microbiol.
Volume:	7
Date:	2016
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-302X
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Physics > Institut für Biologische Physik
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BACTERIAL TRANSFORMATION; STREPTOCOCCUS-PNEUMONIAE; TRANSCRIPTION FACTOR; NATURAL COMPETENCE; DNA UPTAKE; K-STATE; EXPRESSION; COMK; BISTABILITY; EVOLUTION Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27240