Gallas-Lindemann, Carmen ORCID: 0000-0002-6100-4342, Sotiriadou, Isaia, Plutzer, Judit ORCID: 0000-0003-3864-4197, Noack, Michael J., Mahmoudi, Mohammad Reza ORCID: 0000-0001-6820-1288 and Karanis, Panagiotis (2016). Giardia and Cryptosporidium spp. dissemination during wastewater treatment and comparative detection via immunofluorescence assay (IFA), nested polymerase chain reaction (nested PCR) and loop mediated isothermal amplification (LAMP). Acta Trop., 158. S. 43 - 52. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 1873-6254

Full text not available from this repository.

Abstract

Environmental water samples from the Lower Rhine area in Germany were investigated via immunofluorescence assays (IFAs), nested polymerase chain reaction (nested PCR) and loop-mediated isothermal amplification (LAMP) to detect the presence of Giardia spp. (n =185) and Cryptosporidium spp. (n=227). The samples were concentrated through filtration or flocculation, and oocysts were purified via centrifugation through a sucrose density gradient. For all samples, IFA was performed first, followed by DNA extraction for the nested PCR and LAMP assays. Giardia cysts were detected in 105 samples (56.8%) by IFA, 62 samples (33.5%) by nested PCR and 79 samples (42.7%) by LAMP. Cryptosporidium spp. were detected in 69 samples (30.4%) by IFA, 95 samples (41.9%) by nested PCR and 99 samples (43.6%) by LAMP. According to these results, the three detection methods are complementary for monitoring Giardia and Cryptosporidium in environmental waters. (C) 2016 Elsevier B.V. All rights reserved.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Gallas-Lindemann, CarmenUNSPECIFIEDorcid.org/0000-0002-6100-4342UNSPECIFIED
Sotiriadou, IsaiaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Plutzer, JuditUNSPECIFIEDorcid.org/0000-0003-3864-4197UNSPECIFIED
Noack, Michael J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mahmoudi, Mohammad RezaUNSPECIFIEDorcid.org/0000-0001-6820-1288UNSPECIFIED
Karanis, PanagiotisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-275029
DOI: 10.1016/j.actatropica.2016.02.005
Journal or Publication Title: Acta Trop.
Volume: 158
Page Range: S. 43 - 52
Date: 2016
Publisher: ELSEVIER SCIENCE BV
Place of Publication: AMSTERDAM
ISSN: 1873-6254
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
PARVUM OOCYSTS; PROTOZOAN PARASITES; TOXOPLASMA-GONDII; DNA AMPLIFICATION; SAMPLES; SURFACE; DRINKING; GERMANY; IDENTIFICATION; TRANSMISSIONMultiple languages
Parasitology; Tropical MedicineMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/27502

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item