Universität zu Köln

Frerigmann, Henning, Pislewska-Bednarek, Mariola ORCID: 0000-0001-9241-3328, Sanchez-Vallet, Andrea, Molina, Antonio ORCID: 0000-0003-3137-7938, Glawischnig, Erich ORCID: 0000-0001-9280-5065, Gigolashvili, Tamara ORCID: 0000-0002-0416-4796 and Bednarek, Pawel ORCID: 0000-0002-3064-7775 (2016). Regulation of Pathogen-Triggered Tryptophan Metabolism in Arabidopsis thaliana by MYB Transcription Factors and Indole Glucosinolate Conversion Products. Mol. Plant., 9 (5). S. 682 - 696. CAMBRIDGE: CELL PRESS. ISSN 1752-9867

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Abstract

MYB34, MYB51, and MYB122 transcription factors are known as decisive regulators of indolic glucosinolate (IG) biosynthesis with a strong impact on expression of genes encoding CYP79B2 and CYP79B3 enzymes that redundantly convert tryptophan to indole-3-acetaldoxime (IAOx). This intermediate represents a branching point for IG biosynthesis, and pathways leading to camalexin and indole-carboxylic acids (ICA). Here we investigate how these MYBs affect the pathogen-triggered Trp metabolism. Our experiments indicated that these three MYBs affect not only IG production but also constitutive biosynthesis of other IAOx-derived metabolites. Strikingly, the PENETRATION 2 (PEN2)-dependent IG-metabolism products, which are absent in myb34/51/122 and pen2 mutants, were indispensable for full flg22-mediated induction of other IAOx-derived compounds. However, gene induction and accumulation of ICAs and camalexin upon pathogen infection was not compromised in myb34/51/122 plants, despite strongly reduced IG levels. Hence, in comparison with cyp79B2/B3, which lacks all IAOx-derived metabolites, we found myb34/51/122 an ideal tool to analyze IG contribution to resistance against the necrotrophic fungal pathogen Plectosphaerella cucumerina. The susceptibility of myb34/51/122 was similar to that of pen2, but much lower than susceptibility of cyp79B2/B3, indicating that MYB34/51/122 contribute to resistance toward P. cucumerina exclusively through IG biosynthesis, and that PEN2 is the main leaf myrosinase activating IGs in response to microbial pathogens.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Frerigmann, Henning UNSPECIFIED UNSPECIFIED UNSPECIFIED Pislewska-Bednarek, Mariola UNSPECIFIED orcid.org/0000-0001-9241-3328 UNSPECIFIED Sanchez-Vallet, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Molina, Antonio UNSPECIFIED orcid.org/0000-0003-3137-7938 UNSPECIFIED Glawischnig, Erich UNSPECIFIED orcid.org/0000-0001-9280-5065 UNSPECIFIED Gigolashvili, Tamara UNSPECIFIED orcid.org/0000-0002-0416-4796 UNSPECIFIED Bednarek, Pawel UNSPECIFIED orcid.org/0000-0002-3064-7775 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-276048
DOI:	10.1016/j.molp.2016.01.006
Journal or Publication Title:	Mol. Plant.
Volume:	9
Number:	5
Page Range:	S. 682 - 696
Date:	2016
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	1752-9867
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SECONDARY METABOLITES; NONHOST RESISTANCE; CAMALEXIN BIOSYNTHESIS; PLECTOSPHAERELLA-CUCUMERINA; PHYTOALEXIN BIOSYNTHESIS; DISEASE RESISTANCE; DEFENSE; PATHWAY; RESPONSES; PLANTS Multiple languages Biochemistry & Molecular Biology; Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27604