Universität zu Köln

Dedkov, V. G., Lukashev, A. N., Deviatkin, A. A., Kuleshov, K. V., Safonova, M. V., Poleshchuk, M., Drexler, J. F. and Shipulin, G. A. (2016). Retrospective diagnosis of two rabies cases in humans by high throughput sequencing. J. Clin. Virol., 78. S. 74 - 82. AMSTERDAM: ELSEVIER. ISSN 1873-5967

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Abstract

Background: Rabies is prevalent in 150 countries and is definitely found in Russian Federation. The average registered incidence of rabies infection among animals in Russia is 3000 cases per year. At least 500,000 cases of animal bites and scratches are registered in the Russia every year, but only 2-4 cases of rabies infection in humans are reported per year. This relatively low incidence of rabies infection among humans is the result of a well-organized program of rabies surveillance and control as well as the readily available vaccination and immunoglobulin therapies. However, physician awareness of rabies infection in patients with acute encephalopathy is low, and some cases of rabies remain undiagnosed. Objectives: A retrospective study of autopsy materials from patients who died of encephalitis of unknown etiology in the Astrakhan region of Russia in 2003. Study design: A broad-range polymerase chain reaction (PCR) analysis followed by high throughput sequencing were used for the diagnosis. Results: Two cases of rabies were detected and subsequently confirmed using a fluorescent antibody test, an enzyme-linked immunosorbent assay (ELISA) and a mouse inoculation test. Two strains of rabies virus were isolated and characterized using virological methods. The entire genome of each strain was sequenced. (C) 2016 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Dedkov, V. G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lukashev, A. N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Deviatkin, A. A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuleshov, K. V. UNSPECIFIED UNSPECIFIED UNSPECIFIED Safonova, M. V. UNSPECIFIED UNSPECIFIED UNSPECIFIED Poleshchuk, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Drexler, J. F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Shipulin, G. A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-277033
DOI:	10.1016/j.jcv.2016.03.012
Journal or Publication Title:	J. Clin. Virol.
Volume:	78
Page Range:	S. 74 - 82
Date:	2016
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1873-5967
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language REVERSE TRANSCRIPTION-PCR; VIRUS DISCOVERY; IDENTIFICATION; AMPLIFICATION; STRAIN; GENUS; ASSAY Multiple languages Virology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27703