Universität zu Köln

Kleist, Christian, Mohr, Elisabeth, Gaikwad, Sadanand, Dittmar, Laura, Kuerten, Stefanie, Platten, Michael ORCID: 0000-0002-4746-887X, Mier, Walter, Schmitt, Michael, Opelz, Gerhard and Terness, Peter (2016). Autoantigen-specific immunosuppression with tolerogenic peripheral blood cells prevents relapses in a mouse model of relapsing-remitting multiple sclerosis. J. Transl. Med., 14. LONDON: BMC. ISSN 1479-5876

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Abstract

Background: Dendritic cells (DCs) rendered suppressive by treatment with mitomycin C and loaded with the autoantigen myelin basic protein demonstrated earlier their ability to prevent experimental autoimmune encephalomyelitis (EAE), the animal model for multiple sclerosis (MS). This provides an approach for prophylactic vaccination against autoimmune diseases. For clinical application such DCs are difficult to generate and autoantigens hold the risk of exacerbating the disease. Methods: We replaced DCs by peripheral mononuclear cells and myelin autoantigens by glatiramer acetate (Copaxone (R)), a drug approved for the treatment of MS. Spleen cells were loaded with Copaxone (R), incubated with mitomycin C (MICCop) and injected into mice after the first bout of relapsing-remitting EAE. Immunosuppression mediated by MICCop was investigated in vivo by daily assessment of clinical signs of paralysis and in in vitro restimulation assays of peripheral immune cells. Cytokine profiling was performed by enzyme-linked immunosorbent assay (ELISA). Migration of MICCop cells after injection was examined by biodistribution analysis of (111)Indium-labelled MICCop. The number and inhibitory activity of CD4(+)CD25(+)FoxP3(+) regulatory T cells were analysed by histology, flow cytometry and in vitro mixed lymphocyte cultures. In order to assess the specificity of MICCop-induced suppression, treated EAE mice were challenged with the control protein ovalbumin. Humoral and cellular immune responses were then determined by ELISA and in vitro antigen restimulation assay. Results: MICCop cells were able to inhibit the harmful autoreactive T-cell response and prevented mice from further relapses without affecting general immune responses. Administered MICCop migrated to various organs leading to an increased infiltration of the spleen and the central nervous system with CD4(+)CD25(+)FoxP3(+) cells displaying a suppressive cytokine profile and inhibiting T-cell responses. Conclusion: We describe a clinically applicable cell therapeutic approach for controlling relapses in autoimmune encephalomyelitis by specifically silencing the deleterious autoimmune response.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kleist, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Mohr, Elisabeth UNSPECIFIED UNSPECIFIED UNSPECIFIED Gaikwad, Sadanand UNSPECIFIED UNSPECIFIED UNSPECIFIED Dittmar, Laura UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuerten, Stefanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Platten, Michael UNSPECIFIED orcid.org/0000-0002-4746-887X UNSPECIFIED Mier, Walter UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmitt, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Opelz, Gerhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Terness, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-277267
DOI:	10.1186/s12967-016-0860-6
Journal or Publication Title:	J. Transl. Med.
Volume:	14
Date:	2016
Publisher:	BMC
Place of Publication:	LONDON
ISSN:	1479-5876
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language REGULATORY T-CELLS; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS; MITOMYCIN-C; GLATIRAMER ACETATE; DENDRITIC CELLS; DOUBLE-BLIND; STEM-CELLS; IN-VIVO; TRANSPLANTATION; TOLERANCE Multiple languages Medicine, Research & Experimental Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27726