Universität zu Köln

Grosheva, Maria ORCID: 0000-0002-5985-468X, Nohroudi, Klaus, Schwarz, Alisa, Rink, Svenja, Bendella, Habib, Sarikcioglu, Levent ORCID: 0000-0002-0825-6225, Klimaschewski, Lars, Gordon, Tessa and Angelov, Doychin N. (2016). Comparison of trophic factors' expression between paralyzed and recovering muscles after facial nerve injury. A quantitative analysis in time course. Exp. Neurol., 279. S. 137 - 149. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1090-2430

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Abstract

After peripheral nerve injury, recovery of motor performance negatively correlates with the poly-innervation of neuromuscular junctions (NMJ) due to excessive sprouting of the terminal Schwann cells. Denervated muscles produce short-range diffusible sprouting stimuli, of which some are neurotrophic factors. Based on recent data that vibrissal whisking is restored perfectly during facial nerve regeneration in blind rats from the Sprague Dawley (SD)/RCS strain, we compared the expression of brain derived neurotrophic factor (BDNF), fibroblast growth factor-2 (FGF2), insulin growth factors 1 and 2 (IGF1, IGF2) and nerve growth factor (NGF) between SD/RCS and SD-rats with normal vision but poor recovery of whisking function after facial nerve injury. To establish which trophic factors might be responsible for proper NMJ-reinnervation, the transected facial nerve was surgically repaired (facial-facial anastomosis, FFA) for subsequent analysis of mRNA and proteins expressed in the levator labii superioris muscle. A complicated time course of expression included (1) a late rise in BDNF protein that followed earlier elevated gene expression, (2) an early increase in FGF2 and IGF2 protein after 2 days with sustained gene expression, (3) reduced IGF1 protein at 28 days coincident with decline of raised mRNA levels to baseline, and (4) reduced NGF protein between 2 and 14 days with maintained gene expression found in blind rats but not the rats with normal vision. These findings suggest that recovery of motor function after peripheral nerve injury is due, at least in part, to a complex regulation of lesion-associated neurotrophic factors and cytokines in denervated muscles. The increase of FGF-2 protein and concomittant decrease of NGF (with no significant changes in BDNF or IGF levels) during the first week following FFA in SD/RCS blind rats possibly prevents the distal branching of regenerating axons resulting in reduced poly-innervation of motor endplates. (C) 2016 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Grosheva, Maria UNSPECIFIED orcid.org/0000-0002-5985-468X UNSPECIFIED Nohroudi, Klaus UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwarz, Alisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Rink, Svenja UNSPECIFIED UNSPECIFIED UNSPECIFIED Bendella, Habib UNSPECIFIED UNSPECIFIED UNSPECIFIED Sarikcioglu, Levent UNSPECIFIED orcid.org/0000-0002-0825-6225 UNSPECIFIED Klimaschewski, Lars UNSPECIFIED UNSPECIFIED UNSPECIFIED Gordon, Tessa UNSPECIFIED UNSPECIFIED UNSPECIFIED Angelov, Doychin N. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-277349
DOI:	10.1016/j.expneurol.2016.02.020
Journal or Publication Title:	Exp. Neurol.
Volume:	279
Page Range:	S. 137 - 149
Date:	2016
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1090-2430
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FIBROBLAST-GROWTH-FACTOR; RAT SCIATIC-NERVE; TERMINAL SCHWANN-CELLS; ADULT SENSORY NEURONS; DEVELOPING NEUROMUSCULAR-JUNCTIONS; NEUROTROPHIC FACTOR EXPRESSION; FACTOR GENE-EXPRESSION; SPINAL MOTOR-NEURONS; IN-VIVO OBSERVATIONS; FACTOR RECEPTOR 1 Multiple languages Neurosciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27734